CAJ | 학술논문

目的：探讨JC病毒感染与儿童结直肠幼年性息肉之间的关系。方法应用聚合酶链式反应（PCR）及免疫组化PV-9000法检测35例儿童结直肠幼年性息肉和23例儿童正常结直肠黏膜组织中JC病毒DNA片段及T-Ag蛋白。结果 JC病毒DNA片段在儿童结直肠幼年性息肉中的检出率为31.4%（11/35），高于正常结直肠黏膜中的检出率[8.7%（2/23）]；T-Ag蛋白在儿童结直肠幼年性息肉中的阳性表达率为22.9%（8/35），在正常结直肠黏膜中无表达；差异有统计学意义（P均<0.05）。在结直肠幼年性息肉患儿的息肉组织中，JC病毒DNA片段检出率和T-Ag蛋白阳性率与患儿年龄、性别及息肉数量、部位、大小无明显关系（P均>0.05）。结论 JC病毒感染可能与儿童结直肠幼年性息肉有关，JC病毒感染可能是儿童结直肠幼年性息肉形成的危险因素之一。
목적：탐토JC병독감염여인동결직장유년성식육지간적관계。방법응용취합매련식반응（PCR）급면역조화PV-9000법검측35례인동결직장유년성식육화23례인동정상결직장점막조직중JC병독DNA편단급T-Ag단백。결과 JC병독DNA편단재인동결직장유년성식육중적검출솔위31.4%（11/35），고우정상결직장점막중적검출솔[8.7%（2/23）]；T-Ag단백재인동결직장유년성식육중적양성표체솔위22.9%（8/35），재정상결직장점막중무표체；차이유통계학의의（P균<0.05）。재결직장유년성식육환인적식육조직중，JC병독DNA편단검출솔화T-Ag단백양성솔여환인년령、성별급식육수량、부위、대소무명현관계（P균>0.05）。결론 JC병독감염가능여인동결직장유년성식육유관，JC병독감염가능시인동결직장유년성식육형성적위험인소지일。
Objective To investigate the relationship between Jamestown Canyon virus (JCV) infection and colorectal juvenile polyps in children. Methods PCR and immunohistochemical staining were performed to detect JCV DNA sequences and localize T-Ag expression in 35 colorectal juvenile polyps and 23 normal colorectal mucosa. Results The positive rate of JCV gene sequences was 31.4%(11 of 35) in the colorectal juvenile polyps and 8.7%(2 of 23) in the normal colorectal mucosa (P<0.05). T-Ag protein was expressed in 22.9%(8 of 35) of the colorectal juvenile polyps, but none in the normal colorectal mu-cosa (P<0.05). Gender, age, and the number, location, and size of the colorectal juvenile polyps were not significantly different between the children with JCV-positive and JCV-negative polyps (P>0.05). Conclusions This study suggests a positive associ-ation between JCV infection and colorectal juvenile polyps in children, indicating that JCV infection may be a risk factor for colorectal juvenile po-lyps in children.