临床儿科杂志
臨床兒科雜誌
림상인과잡지
2014年
6期
512-516
,共5页
Toll样受体4%Toll样受体7%原发性肾病综合征%儿童
Toll樣受體4%Toll樣受體7%原髮性腎病綜閤徵%兒童
Toll양수체4%Toll양수체7%원발성신병종합정%인동
toll-like receptor 4%toll-like receptor 7%primary nephrofic syndrome%child
目的：探讨Toll样受体（TLR）4、TLR7在儿童原发性肾病综合征（PNS）不同肾脏病理类型中的表达及临床意义。方法纳入110例确诊且经肾活检的PNS患儿，采用免疫组化方法检测肾组织中的TLR4、TLR7，以21例肾母细胞瘤患儿的正常肾组织为对照，分析比较PNS患儿与对照儿童间以及不同病理分型PNS患儿间，肾组织TLR4、TLR7表达的差异。结果不同病理类型PNS患儿和对照组之间肾小管中TLR4、TLR7表达的差异均有统计学意义（P<0.01），其中肾小管TLR4表达水平以MCD型最高，其次MN型、MsPGN型、FSGS型，两两比较差异均有统计学意义（P<0.05）；而肾小管TLR7表达水平以MsPGN型最高，其次为MN型、MCD型、FSGS型，两两比较差异也均有统计学意义（P<0.05）。结论 TLR4、TLR7在PNS患儿肾组织中表达增高，且其表达水平可能与病理类型相关。
目的：探討Toll樣受體（TLR）4、TLR7在兒童原髮性腎病綜閤徵（PNS）不同腎髒病理類型中的錶達及臨床意義。方法納入110例確診且經腎活檢的PNS患兒，採用免疫組化方法檢測腎組織中的TLR4、TLR7，以21例腎母細胞瘤患兒的正常腎組織為對照，分析比較PNS患兒與對照兒童間以及不同病理分型PNS患兒間，腎組織TLR4、TLR7錶達的差異。結果不同病理類型PNS患兒和對照組之間腎小管中TLR4、TLR7錶達的差異均有統計學意義（P<0.01），其中腎小管TLR4錶達水平以MCD型最高，其次MN型、MsPGN型、FSGS型，兩兩比較差異均有統計學意義（P<0.05）；而腎小管TLR7錶達水平以MsPGN型最高，其次為MN型、MCD型、FSGS型，兩兩比較差異也均有統計學意義（P<0.05）。結論 TLR4、TLR7在PNS患兒腎組織中錶達增高，且其錶達水平可能與病理類型相關。
목적：탐토Toll양수체（TLR）4、TLR7재인동원발성신병종합정（PNS）불동신장병리류형중적표체급림상의의。방법납입110례학진차경신활검적PNS환인，채용면역조화방법검측신조직중적TLR4、TLR7，이21례신모세포류환인적정상신조직위대조，분석비교PNS환인여대조인동간이급불동병리분형PNS환인간，신조직TLR4、TLR7표체적차이。결과불동병리류형PNS환인화대조조지간신소관중TLR4、TLR7표체적차이균유통계학의의（P<0.01），기중신소관TLR4표체수평이MCD형최고，기차MN형、MsPGN형、FSGS형，량량비교차이균유통계학의의（P<0.05）；이신소관TLR7표체수평이MsPGN형최고，기차위MN형、MCD형、FSGS형，량량비교차이야균유통계학의의（P<0.05）。결론 TLR4、TLR7재PNS환인신조직중표체증고，차기표체수평가능여병리류형상관。
Objective To study the expression and clinical significance of toll-like receptor (TLR) 4 and TLR7 in primary nephrotic syndrome (PNS) of different pathological types in children. Methods Renal expressions of TLR4 and TLR7 were amined and analyzed retrospectively in renal biopsy specimens from 110 PNS patients and 21 healthy controls by immunohisto-chemical method. According to the renal pathologic classification of PNS, the TLR4 and TLR7 expression levels in PNS of dif-ferent types were compared. Results Compared with the renal tissue of healthy controls, the expression level of TLR4 on renal tissue of PNS patients was significantly increased (P<0.01). Among MN, MsPGN and FSGS, the highest expression of TLR4 was observed in MCD (P<0.01). Compared with the renal tissue of healthy controls, the expression level of TLR7 in re-nal tissue of PNS patients was also significantly increased (P<0.01). Among MCD, MN and FSGS, the highest expression of TLR7 was observed in MsPGN (P<0.01). Conclusions TLR4 and TLR7 expression levels are increased in renal tissue of PNS patients and the expression levels may be correlated with renal pathological types.
