CAJ | 학술논문

目的：建立反相高效液相色谱法测定人血清中丙戊酸钠（VPA）血药浓度的方法学。方法血清酸化后用环戊烷提取，选择环己烷羧酸为内标，以α-溴苯乙酮为衍生化试剂，采用Symmetry C18（250mm ×4.6mm，5μm）分析柱，流动相为乙腈-水（75∶25），检测波长为248nm，流速1mL · min -1，柱温35℃。用高效液相色谱法测定丙戊酸钠血药浓度。结果内标环己烷羧酸和VPA的保留时间分别为10.0min，15.0min，分离效果良好，丙戊酸钠（VPA）血药浓度在6.25～200μg· mL -1范围内呈现良好的线性关系（R＝0.9982），平均回收率98.2％，日间 RSD＜5％。结论本法快速、简便、准确，适合临床常规监测需要。
목적：건립반상고효액상색보법측정인혈청중병무산납（VPA）혈약농도적방법학。방법혈청산화후용배무완제취，선택배기완최산위내표，이α-추분을동위연생화시제，채용Symmetry C18（250mm ×4.6mm，5μm）분석주，류동상위을정-수（75∶25），검측파장위248nm，류속1mL · min -1，주온35℃。용고효액상색보법측정병무산납혈약농도。결과내표배기완최산화VPA적보류시간분별위10.0min，15.0min，분리효과량호，병무산납（VPA）혈약농도재6.25～200μg· mL -1범위내정현량호적선성관계（R＝0.9982），평균회수솔98.2％，일간 RSD＜5％。결론본법쾌속、간편、준학，괄합림상상규감측수요。
OBJECTIVE To determine sodium valproate in patients′serum by high performance liquid chroma-tography ( HPLC ) .METHODS Serum sample ( 100μL ) was extracted with n-pentane after acidifica-tion.Cyclohexanecarboxylic acid and α-bromoacetophenone were selected as an internal standard and derivative rea-gent respectively.The analytical column was Symmetry C 18 (250mm ×4.6mm,5μm) with the temperature at 35℃.The detection wavelength was 248nm and the flow rate was 0.8mL· min-1.The mobile phase consisted of acetoni-trile-water(75∶25).The blood concentration of sodium valproate was determined by HPLC.RESULTS The reten-tion time of internal standard and VPA were 10.0 min and 15.0 mim.The linear range of sodium valproate was 6.25~200mg· L-1,the mean recovery was 98.2%.The relative standard deviation (RSD)of inter-day for sodium val-proate was less than 5%.CONCLUSION The method is rapid ,accurate ,sensitive and suitable for clinica 1 routine monitoring.