海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
6期
147-149
,共3页
超高效液相色谱%环孢素A%血药浓度
超高效液相色譜%環孢素A%血藥濃度
초고효액상색보%배포소A%혈약농도
UPLC%Cyclosporine A%Blood concentration
目的：建立超高效液相色谱法测定全血中环孢素A的质量浓度。方法血样经乙醚萃取后，经WATERS BEH C18（2.1×50mm，1.7μm）分离，乙腈-水为流动相，70℃柱温下进行色谱分析，检测波长为210nm。结果本方法线性范围为0.05～1.0μg· mL -1（r＝0.9930），高、中、低血样的平均回收率分别为94.04％、100.81％、98.95％，日内RSD小于10％。结论本法操作简便，回收率高，精密度好，适用于临床常规检测CsA的血药浓度。
目的：建立超高效液相色譜法測定全血中環孢素A的質量濃度。方法血樣經乙醚萃取後，經WATERS BEH C18（2.1×50mm，1.7μm）分離，乙腈-水為流動相，70℃柱溫下進行色譜分析，檢測波長為210nm。結果本方法線性範圍為0.05～1.0μg· mL -1（r＝0.9930），高、中、低血樣的平均迴收率分彆為94.04％、100.81％、98.95％，日內RSD小于10％。結論本法操作簡便，迴收率高，精密度好，適用于臨床常規檢測CsA的血藥濃度。
목적：건립초고효액상색보법측정전혈중배포소A적질량농도。방법혈양경을미췌취후，경WATERS BEH C18（2.1×50mm，1.7μm）분리，을정-수위류동상，70℃주온하진행색보분석，검측파장위210nm。결과본방법선성범위위0.05～1.0μg· mL -1（r＝0.9930），고、중、저혈양적평균회수솔분별위94.04％、100.81％、98.95％，일내RSD소우10％。결론본법조작간편，회수솔고，정밀도호，괄용우림상상규검측CsA적혈약농도。
OBJECTIVE To establish a UPLC method for detecting cyclosporine A concentration in human whole blood.METHODS CsA was extracted from whole blood with diethyl and separated by a WATERS BEH C 18 (2.1 ×50mm,1.7μm).Mobile phase was a mixture of acetonitrile and water ,the column temperature was set at 70℃,and the detected wavelength was at 210 nm.RESULTS The average linear range of CsA was from 0.05μg· mL-1 to 1.0μg · mL-1 , r =0.9930.The recovery rate of low、moderate、high concentrations were 94.04%、100.81%、98.95% respectively.The intraday RSD were 5.0%( less than 10%) ( n=6 ).CONCLUSION This method is simple ,accurate ,precise and suitable for monitoring blood concentration of CsA.
