CAJ | 학술논문

目的：完善、提高复方益肝灵胶囊质量控制方法。方法采用TLC法鉴别水飞蓟素和五仁醇浸膏；采用HPLC法以甲醇-水（55︰45）、甲醇-水（60︰40）为流动相，在检测波长287nm、250nm处分别测定水飞蓟宾和五味子醇甲。结果薄层色谱斑点清晰，分离度好，专属性强；水飞蓟宾在0.0988～1.976μg（r＝0.9999）线性关系良好，平均加样回收率98.54％，RSD为1.8％；五味子醇甲在0.0951～1.902μg（r＝0.9999）线性关系良好，平均加样回收率98.95％，RSD为1.5％。结论本法简便、可靠、准确，可用于复方益肝灵胶囊的质量控制。
목적：완선、제고복방익간령효낭질량공제방법。방법채용TLC법감별수비계소화오인순침고；채용HPLC법이갑순-수（55︰45）、갑순-수（60︰40）위류동상，재검측파장287nm、250nm처분별측정수비계빈화오미자순갑。결과박층색보반점청석，분리도호，전속성강；수비계빈재0.0988～1.976μg（r＝0.9999）선성관계량호，평균가양회수솔98.54％，RSD위1.8％；오미자순갑재0.0951～1.902μg（r＝0.9999）선성관계량호，평균가양회수솔98.95％，RSD위1.5％。결론본법간편、가고、준학，가용우복방익간령효낭적질량공제。
OBJECTIVE To improve the quality standard for Compound Yiganling Capsules.METHODS Si-lymarin and Extractive material from Fructus Schisandrae Chinensis in Compound Yiganling Capsules were identified by TLC.The silybin and Schisandrol A were determined by HPLC with a mobile phase of methanol -water ( 55∶45 ) and methanol-water (60∶40) respectively.The detection wavelengths were set at 287nm and 250nm.RESULTS The quality identification by TLC was clear ,specific and well separated.The calibration curve of silybin was linear within the range of 0.0988~1.976μg ( r=0.9999 ) , the average recovery rate was 98.54%, RSD was 1.8%.The calibration curve of schisandrol A was linear within the range of 0.0988~1.976μg( r=0.9999 ) ,the average recov-ery rate was 98.95%,RSD was1.5%.CONCLUSION The method is simple,reliable,accurate and can be used for the quality control for Compound Yiganling Capsules.