CAJ | 학술논문

目的：建立党参中党参炔苷的含量测定方法，比较两种不同来源党参的质量。方法采用高效液相色谱法，色谱柱：Syncronis C18（250×4.6 mm，5μm）；流动相：乙腈-0.1％甲酸（24∶76）；流速：1.0mL· min -1；检测波长：269nm。结果党参炔苷在0.019～4.836μg范围内呈良好的线性关系（r＝0.9999），平均回收率100.47％，RSD＝1.17％（n＝5）。应用该方法测得两种不同来源党参中党参炔苷的含量分别为0.33mg· g -1和0.37mg· g -1。结论所建立的方法操作简便、结果准确、重复性好，可以用于党参药材的质量评价。
목적：건립당삼중당삼결감적함량측정방법，비교량충불동래원당삼적질량。방법채용고효액상색보법，색보주：Syncronis C18（250×4.6 mm，5μm）；류동상：을정-0.1％갑산（24∶76）；류속：1.0mL· min -1；검측파장：269nm。결과당삼결감재0.019～4.836μg범위내정량호적선성관계（r＝0.9999），평균회수솔100.47％，RSD＝1.17％（n＝5）。응용해방법측득량충불동래원당삼중당삼결감적함량분별위0.33mg· g -1화0.37mg· g -1。결론소건립적방법조작간편、결과준학、중복성호，가이용우당삼약재적질량평개。
OBJECTIVE To establish the content evaluation method and Compare the quality of two different sources of lobetyolin in Radix Codonopsis.METHODS HPLC was used with chromatographic column:Syncronis C18(250 ×4.6mm,5μm);mobile phase:acetonitrile-0.1% formic acid (24∶76);flow rate:1.0mL· min-1;detec-tion wavelength of 269 nm.RESULTS The linear range of calibration curve was 0.019~4.836μg ( r=0.9999 ) , the average recovery was 100.47%with RSD of 1.17%( n=5 ).The content of obetyolin in two kinds of different sources Radix Codonopsis were 0.33mg· g-1 and 0.37mg· g-1 by this method.CONCLUSION The established method is simple ,accurate and reproducible ,can be used for the quality evaluation of Radix Codonopsis.