CAJ | 학술논문

利用基于原子力显微镜(AFM)的力谱技术,在正常生长的单个活细胞表面上,实时动态地研究了免疫毒素 LHRH-PE40对 HeLa 细胞表面硬度的影响。采用 Hertz-Sneddon 模型计算所得力曲线相应的杨氏模量。实验表明,LHRH-PE40会引发 HeLa 细胞表面硬度逐步增加,且这种硬度的增加与细胞内微丝骨架的重组聚集有关系。本研究为全面掌握 LHRH-PE40的药用效果和作用机理提供了重要信息。
이용기우원자력현미경(AFM)적력보기술,재정상생장적단개활세포표면상,실시동태지연구료면역독소 LHRH-PE40대 HeLa 세포표면경도적영향。채용 Hertz-Sneddon 모형계산소득력곡선상응적양씨모량。실험표명,LHRH-PE40회인발 HeLa 세포표면경도축보증가,차저충경도적증가여세포내미사골가적중조취집유관계。본연구위전면장악 LHRH-PE40적약용효과화작용궤리제공료중요신식。
The quantitative analysis of biomechanical profiles at the single-cell level can provide additional information. It is usually not available in traditional cell biology approaches, but may be crucial to assess and understand tumor prognosis and response to chemotherapy. In this study, the on-line changes of cell elastic properties after the addition of cancer target drug LHRH-PE40 were monitored by atomic force microscopy ( AFM) on living HeLa cell surface under physiological condition. The results from AFM based force spectroscopy showed that LHRH-PE40 induced a distinct increase of the cell surface elasticity of HeLa cells. The fluorescence images implied that the target drug LHRH-PE40 would affect the re-organization of cell actions, which led to the increase of the elasticity of HeLa cells.