分析化学
分析化學
분석화학
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2014年
6期
799-804
,共6页
丁良%杨慧%席亚楠%张金超%申世刚
丁良%楊慧%席亞楠%張金超%申世剛
정량%양혜%석아남%장금초%신세강
纳米簇%阳离子交换%荧光%信号放大%免疫测定
納米簇%暘離子交換%熒光%信號放大%免疫測定
납미족%양리자교환%형광%신호방대%면역측정
Nano clusters%Cation exchange%Fluorescence%Ssignal amplification%Immunoassay
利用非荧光硫化锌纳米簇(NCCs)阳离子交换(CX)反应检测痕量生物分子。水热法合成的纳米簇是多孔的,可以通过快速阳离子交换反应从纳米簇中释放大量的 Zn2+,在锌响应试剂的作用下产生荧光信号,进行荧光检测。当纳米簇的平均直径分别为44,86和144 nm 时,研究了 Zn2+的释放效率和目标结合力与平均直径之间的关系。结果表明,最小的纳米簇表现出最高的阳离子交换效率,71%被封闭的 Zn2+可以在2 min 内通过微波辐射释放出来。当使用44 nm 纳米簇夹心法测定免疫球蛋白 E(IgE)时,检出限为5 ng/ L,比 ELISA 法低1000倍。结果表明,利用硫化锌纳米簇的阳离子交换作用,所得结果在高扩增效率、稳定性和生物相容性方面优于传统的信号方法。
利用非熒光硫化鋅納米簇(NCCs)暘離子交換(CX)反應檢測痕量生物分子。水熱法閤成的納米簇是多孔的,可以通過快速暘離子交換反應從納米簇中釋放大量的 Zn2+,在鋅響應試劑的作用下產生熒光信號,進行熒光檢測。噹納米簇的平均直徑分彆為44,86和144 nm 時,研究瞭 Zn2+的釋放效率和目標結閤力與平均直徑之間的關繫。結果錶明,最小的納米簇錶現齣最高的暘離子交換效率,71%被封閉的 Zn2+可以在2 min 內通過微波輻射釋放齣來。噹使用44 nm 納米簇夾心法測定免疫毬蛋白 E(IgE)時,檢齣限為5 ng/ L,比 ELISA 法低1000倍。結果錶明,利用硫化鋅納米簇的暘離子交換作用,所得結果在高擴增效率、穩定性和生物相容性方麵優于傳統的信號方法。
이용비형광류화자납미족(NCCs)양리자교환(CX)반응검측흔량생물분자。수열법합성적납미족시다공적,가이통과쾌속양리자교환반응종납미족중석방대량적 Zn2+,재자향응시제적작용하산생형광신호,진행형광검측。당납미족적평균직경분별위44,86화144 nm 시,연구료 Zn2+적석방효솔화목표결합력여평균직경지간적관계。결과표명,최소적납미족표현출최고적양리자교환효솔,71%피봉폐적 Zn2+가이재2 min 내통과미파복사석방출래。당사용44 nm 납미족협심법측정면역구단백 E(IgE)시,검출한위5 ng/ L,비 ELISA 법저1000배。결과표명,이용류화자납미족적양리자교환작용,소득결과재고확증효솔、은정성화생물상용성방면우우전통적신호방법。
Cation exchange (CX) reaction for the non-fluorescent ZnS nanocrystal clusters (NCCs) can be used to detect trace biomolecules . Nano clusters synthetized by hydrothermal synthesis are porous. So they can quickly release large amounts of Zn2+ from through cation exchange ( CX) reaction and nano cluster, generate fluorescent signal under the action of zinc reagent to detect fluorescence. The relationship between the release efficiency, target binding force of Zn2+ and its average diamete was investigated when the average diameter was 44 nm, 86 nm and 144 nm in this experiment. Results showed that the smallest nano cluster exhibited the highest cation exchange efficiency, and 71 percent of Zn2+ closed could be released by microwave radiation within 2 min. When the sandwich method of NCCs of 44-nm was used to detect immunoglobulin E (IgE) in a sandwich assay, the limit of detection (LOD) was 5 ng / L, which was 1000 times lower than that of ELISA. It turns out that CX for the ZnS NCCs is superior to the conventional signaling strategies in its high amplification efficiency, robustness, and biocompatibility.