CAJ | 학술논문

建立了一种用于多重细菌鉴定的微流控芯片分析方法。在芯片上实现细菌进样、培养和鉴定，结合培养池阵列的空间分辨力以及菌种特异性显色培养基的颜色分辨力，可以实现多重细菌检测。实验选用4种泌尿系统感染常见病原菌作为模拟测试对象，结果显示，这种芯片方法在15 h 内可完成细菌鉴定，检测限可达101 cfu/ mL。临床样本测试结果显示，芯片方法可以实现4种细菌同步鉴定，检测结果与传统方法的一致性达到96.3%。这种微流控细菌鉴定方法简便快速，有望发展成为细菌检测的有力工具。
건립료일충용우다중세균감정적미류공심편분석방법。재심편상실현세균진양、배양화감정，결합배양지진렬적공간분변력이급균충특이성현색배양기적안색분변력，가이실현다중세균검측。실험선용4충비뇨계통감염상견병원균작위모의측시대상，결과현시，저충심편방법재15 h 내가완성세균감정，검측한가체101 cfu/ mL。림상양본측시결과현시，심편방법가이실현4충세균동보감정，검측결과여전통방법적일치성체도96.3%。저충미류공세균감정방법간편쾌속，유망발전성위세균검측적유력공구。
We developed a microfluidic device to integrate sample introduction, bacteria culturing and results reading. The identification of multiple bacteria was achieved by combining the spatial resolution of the arrayed bacteria culture chambers and the color resolution benefited from the bacteria specific chromogenic media. A set of 4 common pathogenic bacteria responsible for urinary tract infection were used as a model to test the microfluidic assay. Our results showed that the bacteria identification assay can be completed in 15 h, with a limit of detection (LOD) of bacteria density down to 10 cfu / mL. Clinical sample testing using the microchip approach showed a coincidence rate of 96. 3% as compared with the conventional method. The developed microfluidic approach is simple and rapid, thus hold the potential to serve as a powerful tool for detection of multiple bacteria.