CAJ | 학술논문

对杨树不同亲和性授粉过程中（加杨×加杨，加杨×银腺杨）花粉的萌发及花粉管的伸长进行了荧光染色观察，并对雌蕊的内源性激素及保护性酶的含量进行分析。结果表明，加杨×银腺杨授粉后花粉管伸长明显慢于加杨×加杨，同时，授粉均引发了柱头保护性酶SOD和POD的波动，但两种授粉所引发的酶活性变化不同，加杨×加杨授粉后SOD活性升高要早于加杨×银腺杨，而加杨×银腺杨授粉后POD活性在增加过程中有下降的变化。加杨×加杨授粉后雌蕊内源激素生长素（ IAA）、赤霉素（ GA3）及玉米核苷素（ ZR）等质量分数要高于加杨×银腺杨，而ABA质量分数远低于加杨×银腺杨。结果表明，SOD活性的延迟升高，低水平的IAA、GA3、ZR 以及高水平的ABA可能对授粉后花粉管的伸长起抑制作用，是引发杨树杂交授粉不亲和性的原因之一。
대양수불동친화성수분과정중（가양×가양，가양×은선양）화분적맹발급화분관적신장진행료형광염색관찰，병대자예적내원성격소급보호성매적함량진행분석。결과표명，가양×은선양수분후화분관신장명현만우가양×가양，동시，수분균인발료주두보호성매SOD화POD적파동，단량충수분소인발적매활성변화불동，가양×가양수분후SOD활성승고요조우가양×은선양，이가양×은선양수분후POD활성재증가과정중유하강적변화。가양×가양수분후자예내원격소생장소（ IAA）、적매소（ GA3）급옥미핵감소（ ZR）등질량분수요고우가양×은선양，이ABA질량분수원저우가양×은선양。결과표명，SOD활성적연지승고，저수평적IAA、GA3、ZR 이급고수평적ABA가능대수분후화분관적신장기억제작용，시인발양수잡교수분불친화성적원인지일。
We obsevered pollen germination and pollen tube growth after pollinations ( Populus Canadensis Moench ×P.Canaden-sis Moench and P.canadensis Moench×P.alba×P.glandulosa) with a fluorescence microscope, and analyzed the content of endogenous hormones and protective enzymes of pistils.The pollen tubes of P.canadensis Moench×P.alba×P.glandu-losa samples grow significantly slower than that of the P.canadensis Moench×P.canadensis Moench, and different pollina-tion combination initiated the coordinated variation of content of protective enzymes and endogenous hormones in pistil.The SOD activity in P.canadensis Moench ×P.canadensis Moench increases earlier than that of P.canadensis Moench ×P.al-ba×P.glandulosa, whereas, the POD activity of the latter shows a downward change during the process of ascent.Hor-mones including IAA, GA3 and ZR are higher in P.canadensis Moench×P.canadensis, while the ABA content is much lower than that in P.canadensis Moench ×P.alba×P.glandulosa.The delay of SOD activityincrease , the low contents of IAA, GA3, ZR and high concent of ABA are possible reasons on leading to cross-pollination incompatibilityof poplar.