中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
7期
89-91,92
,共4页
筋骨草%乙酰哈巴苷%哈巴苷%高效液相色谱法%蒸发光散射检测器%含量测定
觔骨草%乙酰哈巴苷%哈巴苷%高效液相色譜法%蒸髮光散射檢測器%含量測定
근골초%을선합파감%합파감%고효액상색보법%증발광산사검측기%함량측정
Ajuga decumbens Thunb.%8-O-acetylharpagide%harpagide%HPLC%ELSD%content determination
目的：建立高效液相色谱-蒸发光散射检测器法(HPLC-ELSD)同时测定筋骨草药材中乙酰哈巴苷和哈巴苷含量的方法,并对不同产地和批次的筋骨草药材进行测定。方法采用Dikma C18色谱柱(4.6 mm×150 mm,5μm)；流动相A为水,B为乙腈,梯度洗脱(0～6 min,5%B；6～11 min,5%～15%B；11～17 min,15%B)；洗脱流速1 mL/min；柱温30℃。蒸发光散射检测器漂移管温度103℃,以压缩空气为雾化气,气体流速1.6 L/min。结果乙酰哈巴苷在0.017～17.32μg、哈巴苷在0.003～1.62μg范围内呈良好线性关系,精密度和准确性良好。福建6个批号样品的乙酰哈巴苷和哈巴苷含量较高。结论乙酰哈巴苷和哈巴苷可作为监测筋骨草药材质量的指标性成分。
目的：建立高效液相色譜-蒸髮光散射檢測器法(HPLC-ELSD)同時測定觔骨草藥材中乙酰哈巴苷和哈巴苷含量的方法,併對不同產地和批次的觔骨草藥材進行測定。方法採用Dikma C18色譜柱(4.6 mm×150 mm,5μm)；流動相A為水,B為乙腈,梯度洗脫(0～6 min,5%B；6～11 min,5%～15%B；11～17 min,15%B)；洗脫流速1 mL/min；柱溫30℃。蒸髮光散射檢測器漂移管溫度103℃,以壓縮空氣為霧化氣,氣體流速1.6 L/min。結果乙酰哈巴苷在0.017～17.32μg、哈巴苷在0.003～1.62μg範圍內呈良好線性關繫,精密度和準確性良好。福建6箇批號樣品的乙酰哈巴苷和哈巴苷含量較高。結論乙酰哈巴苷和哈巴苷可作為鑑測觔骨草藥材質量的指標性成分。
목적：건립고효액상색보-증발광산사검측기법(HPLC-ELSD)동시측정근골초약재중을선합파감화합파감함량적방법,병대불동산지화비차적근골초약재진행측정。방법채용Dikma C18색보주(4.6 mm×150 mm,5μm)；류동상A위수,B위을정,제도세탈(0～6 min,5%B；6～11 min,5%～15%B；11～17 min,15%B)；세탈류속1 mL/min；주온30℃。증발광산사검측기표이관온도103℃,이압축공기위무화기,기체류속1.6 L/min。결과을선합파감재0.017～17.32μg、합파감재0.003～1.62μg범위내정량호선성관계,정밀도화준학성량호。복건6개비호양품적을선합파감화합파감함량교고。결론을선합파감화합파감가작위감측근골초약재질량적지표성성분。
Objective To build an HPLC-ELSD method for simultaneous determination of 8-O-acetylharpagide and harpagide inAjuga decumbens Thunb.;To determineAjuga decumbens Thunb. samples from different area and of different batches.Methods Chromatographic separation was achieved on a Dikma C18 column (4.6 mm×150 mm, 5μm). The mobile phase consisted of water (A) and acetonitrile (B), using a gradient elution of 5% B at 0-6 min, 5%-15% B at 6-11 min, 15% B at 11-17 min, and the flow rate was 1.0 mL/min. An evaporative light scattering-detector (ELSD) was used with the temperature of drift tube at 103℃ and the gas flow rate of air was at 1.6 L/min.Results Good linearity was shown at the concentration range of 0.017-17.32μg for 8-O-acetylharpagide, and 0.003-1.62μg for harpagide. The validated method was of great precision and accuracy. The contents of 8-O-acetylharpagide and harpagide inAjuga decumbens Thunb. from Fujian province were higher than the contents in other localities.Conclusion The content of harpagide can be used as index component to monitor quality control ofAjuga decumbens Thunb..
