CAJ | 학술논문

目的：观察艾烟可吸入颗粒物(PM10)对人肺腺癌A549细胞凋亡的影响,并探讨其诱导凋亡的可能机制。方法采用体外实验方法,以 A549细胞为研究对象,采用 Hoechest33258染色法,荧光显微镜观察艾烟PM10对A549细胞凋亡的影响,检测细胞内Ca2+水平、核因子(NF)-κB p65表达量及细胞内活性氧(ROS)的水平。结果艾烟PM10处理4 h在400μg/mL时出现部分凋亡细胞。艾烟PM10干预A549细胞4 h后,随着浓度增加,细胞内Ca2+水平显著增加(P＜0.01)。与空白对照组比较,艾烟干预4 h,各浓度组A549细胞内NF-κB p65表达量均降低(P＜0.05)；干预20 h,70μg/mL组和280μg/mL组NF-κB p65表达量均降低(P＜0.05),140μg/mL组NF-κB p65含量无明显变化(P＞0.05)。相同浓度下,不同时点艾烟干预各组间比较,NF-κB p65表达量无明显变化(P＞0.05)。与空白对照组比较,艾烟PM10处理各组细胞内ROS水平均显著降低(P＜0.05)。结论艾烟PM10能诱导A549细胞凋亡、提高A549细胞内Ca2+水平。
목적：관찰애연가흡입과립물(PM10)대인폐선암A549세포조망적영향,병탐토기유도조망적가능궤제。방법채용체외실험방법,이 A549세포위연구대상,채용 Hoechest33258염색법,형광현미경관찰애연PM10대A549세포조망적영향,검측세포내Ca2+수평、핵인자(NF)-κB p65표체량급세포내활성양(ROS)적수평。결과애연PM10처리4 h재400μg/mL시출현부분조망세포。애연PM10간예A549세포4 h후,수착농도증가,세포내Ca2+수평현저증가(P＜0.01)。여공백대조조비교,애연간예4 h,각농도조A549세포내NF-κB p65표체량균강저(P＜0.05)；간예20 h,70μg/mL조화280μg/mL조NF-κB p65표체량균강저(P＜0.05),140μg/mL조NF-κB p65함량무명현변화(P＞0.05)。상동농도하,불동시점애연간예각조간비교,NF-κB p65표체량무명현변화(P＞0.05)。여공백대조조비교,애연PM10처리각조세포내ROS수평균현저강저(P＜0.05)。결론애연PM10능유도A549세포조망、제고A549세포내Ca2+수평。
Objective To observe the effect of PM10 (inhalable particles) in moxa smoke on apoptosis of human lung adenocarcinoma cell line-A549 cells, and explore the possible mechanisms of inducing apoptosisMethods The A549 cells were studied in vitro experiment method, which were stained by Hoechest33258 staining method. Their morphological changes of apoptosis were observed by the fluorescence microscopy. The levels of intracellular Ca2+ and reactive oxygen species (ROS), and expression of NF-κB p65 were also measured.Results Some apoptotic cells were observed after treated with moxa smoke PM10 in the concerntration of 400μg/mL. After 4 hours intervention by moxa smoke PM10 in A549 cells, the intracellular Ca2+ level increased significantly (P<0.01). Compared with the blank control group, the expression of NF-κB p65 decreased significantly (P<0.05) after intervention of moxa smoke PM10 with different concerntrations for 4 hours. When the A549 cells were cultured with moxa smoke PM10 for 20 hours, the expression of p65 decreased significantly (P<0.05) in the concerntrations of 70, 280μg/mL, while there was no significant change in the concerntration of 140μg/mL (P>0.05). Compared with the blank control group, ROS level was significantly lower (P<0.05) in A549 cells after intervention of moxa smoke PM10.Conclusion PM10 in moxa smoke could induce apoptosis of A549 cells, could increase cytosolic Ca2+ level.