CAJ | 학술논문

目的：观察抗衰益智胶囊对衰老模型大鼠脑组织三磷酸腺苷(ATP)酶活性和血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的影响,探讨其对脑组织的保护作用。方法受试动物按随机数字表法分为空白组和造模组。采用D-半乳糖腹腔注射造模,造模成功的大鼠分为模型组、脑复康组和抗衰益智胶囊高、低剂量组,各给药组给予相应药物灌胃,正常组和模型组每日给予等量生理盐水灌胃,连续60 d,测定各组大鼠脑组织 Na+-K+-ATP 酶、Ca2+-Mg2+-ATP 酶和血清 IL-6、TNF-α含量。结果与空白组比较,模型组大鼠脑组织Na+-K+-ATP 酶和 Ca2+-Mg2+-ATP 酶活性降低,血清 IL-6和 TNF-α含量增高,差异有统计学意义(P＜0.05,P＜0.01)；与模型组比较,各给药组大鼠脑组织Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性升高,血清IL-6和TNF-α含量降低,差异有统计学意义(P＜0.05),且以抗衰益智胶囊高剂量组作用为优。结论抗衰益智胶囊具有提高衰老大鼠脑组织ATP酶活性和降低促炎因子水平的作用。
목적：관찰항쇠익지효낭대쇠로모형대서뇌조직삼린산선감(ATP)매활성화혈청백세포개소-6(IL-6)、종류배사인자-α(TNF-α)적영향,탐토기대뇌조직적보호작용。방법수시동물안수궤수자표법분위공백조화조모조。채용D-반유당복강주사조모,조모성공적대서분위모형조、뇌복강조화항쇠익지효낭고、저제량조,각급약조급여상응약물관위,정상조화모형조매일급여등량생리염수관위,련속60 d,측정각조대서뇌조직 Na+-K+-ATP 매、Ca2+-Mg2+-ATP 매화혈청 IL-6、TNF-α함량。결과여공백조비교,모형조대서뇌조직Na+-K+-ATP 매화 Ca2+-Mg2+-ATP 매활성강저,혈청 IL-6화 TNF-α함량증고,차이유통계학의의(P＜0.05,P＜0.01)；여모형조비교,각급약조대서뇌조직Na+-K+-ATP매화Ca2+-Mg2+-ATP매활성승고,혈청IL-6화TNF-α함량강저,차이유통계학의의(P＜0.05),차이항쇠익지효낭고제량조작용위우。결론항쇠익지효낭구유제고쇠로대서뇌조직ATP매활성화강저촉염인자수평적작용。
Objective To observe effects of Kangshuai Yizhi Capsule on ATP in brain tissue and IL-6, TNF-α in serum of aging model rats, and explore the protective effects of the capsule on brain tissue.Methods Totally 72 rats were randomly divided into a normal group and a model group. The subacutely aging model rats were made by injectingD-gal, then aging rats were numbered and grouped by random number table into the model group, Kangshuai Yizhi high-dose group, Kangshuai Yizhi Capsule low-dose group and Naofukang group. All dose groups were received gavage by giving corresponding doses, while normal group and model group were given the same amount of saline everyday. After treated for 60 days, the activity of Na+-K+-ATP and Ca2+-Mg2+-ATP in brain tissue, and IL-6, TNF-α in serum were detected.Results Compared with normal group, Na+-K+-ATP and Ca2+-Mg2+-ATP were less active (P<0.05), but levels of IL-6 and TNF-α in model group were significantly higher, with statistical significance (P<0.05). Compared with model group, after treated with Kangshuai Yizhi Capsule, Na+-K+-ATP and Ca2+-Mg2+-ATP were more active, and IL-6 and TNF-α levels were down-regulated significantly in dose groups, with statistical significance (P<0.05). Meanwhile, Kangshuai Yizhi Capsule high-dose group showed the most obvious effect among dose groups.ConclusionKangshuai Yizhi Capsule has effects of enhancing activity of ATP in brain tissue and reducing level of proinflammatory factors.