CAJ | 학술논문

目的：观察竹节参总提物对衰老小鼠的学习记忆、抗氧化及抗凋亡的影响,并探讨其作用机制。方法雄性昆明种小鼠随机分为正常组、模型组、维生素E组和竹节参低、高剂量组。除正常组外,其余各组颈背部皮下注射D-半乳糖建立衰老模型。各给药组分别给予竹节参总提物和维生素E灌胃,正常组和模型组给予等量生理盐水灌胃,1次/d,连续7周。第8周进行小鼠学习和记忆能力测试。测试结束后,苏木素-伊红染色观察海马CA1区神经元的形态变化,生化法检测脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)水平,RT-PCR检测脑组织中Bcl-2、Bax mRNA表达。结果竹节参低、高剂量组均能缩短小鼠寻找平台时间,改善小鼠学习记忆能力；显著增强SOD、GSH-Px活性,降低MDA含量；升高Bcl-2 mRNA、降低Bax mRNA表达水平。结论竹节参总提物具有抗衰老的作用。
목적：관찰죽절삼총제물대쇠로소서적학습기억、항양화급항조망적영향,병탐토기작용궤제。방법웅성곤명충소서수궤분위정상조、모형조、유생소E조화죽절삼저、고제량조。제정상조외,기여각조경배부피하주사D-반유당건립쇠로모형。각급약조분별급여죽절삼총제물화유생소E관위,정상조화모형조급여등량생리염수관위,1차/d,련속7주。제8주진행소서학습화기억능력측시。측시결속후,소목소-이홍염색관찰해마CA1구신경원적형태변화,생화법검측뇌조직초양화물기화매(SOD)、병이철(MDA)화곡광감태과양화물매(GSH-Px)수평,RT-PCR검측뇌조직중Bcl-2、Bax mRNA표체。결과죽절삼저、고제량조균능축단소서심조평태시간,개선소서학습기억능력；현저증강SOD、GSH-Px활성,강저MDA함량；승고Bcl-2 mRNA、강저Bax mRNA표체수평。결론죽절삼총제물구유항쇠로적작용。
Objective To research the protective effects of totalPanax Japonicus extract on learning memory, antioxidation, and anti-apoptosis of aging mice,and explore the mechanism. Methods Male mice were randomly divided into normal group, model group, Vitamin E (VE) group, Panax Japonicus extract low and high dose group. Except for the normal group, the other groups were injected withD-gal on the back of the neck subcutaneously to establish aging model. Normal group and model group were given a gavage with saline and each treatment group was given a gavage with totalPanax Japonicus extract and VE once a day for 7 weeks after the aging model established. All mice were be measured their learning and memory ability in the eighth week. After the test, the morphological changes of CA1 neurons were observed by HE stain. SOD, GSH-Px, MDA levels in brain tissue were measured by biochemical method, the expressions of Bcl-2 and Bax mRNA were evaluated by RT-PCR.Results Mice inPanax Japonicus extract low and high dose group could spend less time in searching for the platform, improve the learning and memory ability. TotalPanax Japonicus extract increased the activity of SOD and GSH-Px, while decreased the content of MDA. In addition, it could increase the expression level of Bcl-2 mRNA and reduce the expression level of Bax mRNA as well.Conclusion TotalPanax Japonicus extract has anti-aging effect.