CAJ | 학술논문

基于核酸外切酶I选择性消化单链核酸的特点,使用MCF-7细胞表面过量表达的肿瘤标志蛋白MUC1的适体,构建了一种灵敏检测乳腺癌细胞的新型电化学传感器。核酸适体链与乳腺癌细胞MCF-7表面过量表达的肿瘤标志蛋白MUC1的结合会阻碍其与互补核酸探针链的杂交,所以电极表面固定的未杂交的核酸探针单链就会被外切酶I选择性消化从而失去末端的亚甲基蓝信号分子。因此,通过检测电化学信号的变化,此传感器在103~106 cell/mL细胞浓度范围内线性检测乳腺癌细胞MCF-7,检出限为330 cell/mL,具有高度特异性,可以有效区分对照细胞胰岛β细胞。
기우핵산외절매I선택성소화단련핵산적특점,사용MCF-7세포표면과량표체적종류표지단백MUC1적괄체,구건료일충령민검측유선암세포적신형전화학전감기。핵산괄체련여유선암세포MCF-7표면과량표체적종류표지단백MUC1적결합회조애기여호보핵산탐침련적잡교,소이전겁표면고정적미잡교적핵산탐침단련취회피외절매I선택성소화종이실거말단적아갑기람신호분자。인차,통과검측전화학신호적변화,차전감기재103~106 cell/mL세포농도범위내선성검측유선암세포MCF-7,검출한위330 cell/mL,구유고도특이성,가이유효구분대조세포이도β세포。
In terms of the characteristic of exonuclease I that can selectively catalyze the degradation of single-stranded DNA, we report a new sensitive electrochemical biosensor for the detection of breast cancer cells by using the aptamers of MUC1 that is overexpressed on the surface of MCF-7 cells. Because the binding of aptamer and MUC1 that is over-expressed on the surface of breast cancer cell MCF-7 can prevent its hybridization with the complementary probe DNA, the unhybridized single-stranded probe DNA that is immobilized on the electrode surface will be selectively digested by Exonuclease I and result in the removal of the signal molecule methylene blue. Therefore, by tracing the changes in electrochemical responses, the biosensor can be used to sensitively detect MCF-7 cells in a linear range from 103 to 106 cell/mL with a detection limit of 330 cell/mL, and easily distinguish the control islet β cells with high specificity.