CAJ | 학술논문

利用自组装膜技术在石英片表面构建了多肽自组装膜。考察了组装液浓度和组装时间对多肽自组装膜组装效果的影响,并用紫外可见吸收光谱仪和扫描电镜对该膜进行了表征。研究结果表明,当γ-氨基丙基三乙氧基硅烷水溶液浓度为1%(V/V),组装时间为3 h；金纳米浓度为2.4×10-4 mol/L,组装时间为12 h；多肽浓度为1×10-4 mol/L,组装时间为12 h时,组装效果最好。基于凝血酶可特异性酶切多肽中精氨酸和甘氨酸之间的肽键,建立了一种测定微量凝血酶的新方法,方法的线性响应范围为2.8×10-12~9.9×10-10 mol/L,检出限为1.4×10-12 mol/L。此膜用于血清样品中凝血酶的测定,回收率为91.6%~107.6%。
이용자조장막기술재석영편표면구건료다태자조장막。고찰료조장액농도화조장시간대다태자조장막조장효과적영향,병용자외가견흡수광보의화소묘전경대해막진행료표정。연구결과표명,당γ-안기병기삼을양기규완수용액농도위1%(V/V),조장시간위3 h；금납미농도위2.4×10-4 mol/L,조장시간위12 h；다태농도위1×10-4 mol/L,조장시간위12 h시,조장효과최호。기우응혈매가특이성매절다태중정안산화감안산지간적태건,건립료일충측정미량응혈매적신방법,방법적선성향응범위위2.8×10-12~9.9×10-10 mol/L,검출한위1.4×10-12 mol/L。차막용우혈청양품중응혈매적측정,회수솔위91.6%~107.6%。
Peptide self-assembled multilayers ( SAMs ) was coated onto the quartz surface. The assembly conditions, such as the assembly agent concentration and assembly time, were examined. The SAMs was characterized via UV-vis absorption spectrometry and scanning electron microscope. Our results showed that the optimal concentration and assembly time for the 3-aminopropyl-triethoxysilane aqueous were 1% ( V/V ) and 3 h respectively, and those of gold nanoparticles were 2. 4 × 10-4 mol/L and 12 h, respectively, while those of peptide solution were 1 × 10-4 mol/L and 12 h, respectively. A new sensitive method, based on the theory that peptide could be cleaved at the site of Arg-Gly by thrombin, was established to detect thrombin. In addition, a good linear relationship was obtained in the range from 2. 8×10-12 mol/L to 9. 9×10-10 mol/L, and the detection limit was 1. 4×10-12 mol/L. The peptide self-assembled multilayers were also used in the analysis of blood serum samples, and the recovery rate was within the range from 91. 6% to 107. 6%.