CAJ | 학술논문

利用纳米金的表面增强拉曼效应,建立了一种快速、灵敏、无标记、无分离的检测三聚氰胺的分析方法。通过柠檬酸钠还原法制得平均粒径为30 nm的纳米金溶胶,利用Au-S之间的共价键作用,将对巯基苯硼酸(4-MPBA)自组装到纳米金的表面,构建了一个三聚氰胺的检测平台。当溶液中存在三聚氰胺( MA)时, MA与4-MPBA之间存在强烈的氢键作用,使4-MPBA功能化的纳米金发生聚集。而且,MA的浓度越高,纳米金的聚集程度越大,形成的“热点”越多,4-MPBA和MA的拉曼信号越强。4-MPBA与MA的拉曼特征峰分别位于1076和715 cm-1处,若以I715与I1076的比值为依据,便可以实现三聚氰胺的定性及定量的检测,线性检测范围为0.1~1.5μmol/L,检出限(LOD)为0.02μmol/L。
이용납미금적표면증강랍만효응,건립료일충쾌속、령민、무표기、무분리적검측삼취청알적분석방법。통과저몽산납환원법제득평균립경위30 nm적납미금용효,이용Au-S지간적공개건작용,장대구기분붕산(4-MPBA)자조장도납미금적표면,구건료일개삼취청알적검측평태。당용액중존재삼취청알( MA)시, MA여4-MPBA지간존재강렬적경건작용,사4-MPBA공능화적납미금발생취집。이차,MA적농도월고,납미금적취집정도월대,형성적“열점”월다,4-MPBA화MA적랍만신호월강。4-MPBA여MA적랍만특정봉분별위우1076화715 cm-1처,약이I715여I1076적비치위의거,편가이실현삼취청알적정성급정량적검측,선성검측범위위0.1~1.5μmol/L,검출한(LOD)위0.02μmol/L。
A rapid, sensitive, label-free and separation-free analytical method for determination of melamine ( MA) was developed based on surface enhanced Raman scattering ( SERS ) effect of gold nanoparticles. Through tri-sodium citrate reduction method, gold nanoparticles with average diameter of 30 nm were obtained. The melamine detection platform was constructed after self-assembling 4-mercapto phenylboronic acid (4-MPBA) on the surface of gold nanoparticles through Au S covalent bond. When MA existed in solution, 4-MPBA functionalized gold nanoparticles would aggregate because of strong hydrogen bond interaction between MA and 4-MPBA. Moreover, following increase of the concentration of MA, gold nanoparticles would aggregate more intensively and form more "hot spots". As a result, Raman signal of 4-MPBA and MA was enhanced greatly. The characteristic Raman peaks of 4-MPBA and MA respectively located at 1076 cm-1 and 715 cm-1 . Hence, the qualitative and quantitative detection for MA were realized based on the ratio value of I715 cm-1 to I1076 cm-1 . The linear range of MA detection was 0 . 1 μmol/L-1. 5 μmol/L. The limit of detection (LOD) reached 0. 02 μmol/L in terms of three times signal to noise.