CAJ | 학술논문

采用柠檬酸钠还原法合成了粒径为13 nm 的金纳米粒子,并在其表面修饰上谷胱甘肽( GSH-AuNPs)。在一定的盐浓度范围内,谷胱甘肽能保护金纳米粒子免受盐诱导的聚集。当神经元素3( Neuroge-nin3,ngn3)多肽片段存在时,在一定的盐浓度下,ngn3片段能够诱导GSH-AuNPs发生聚集,使金纳米粒子溶液由红色变成蓝色。以谷胱甘肽修饰的金纳米粒子为探针,建立了快速检测ngn3片段的比色方法。通过优化得到的最适实验条件为：ngn3与 GSH-AuNPs 的平衡反应时间10 min,缓冲液pH=6.0, NaCl 浓度100 mmol/L。在优化条件下,检测ngn3的线性范围为20~300μg/L,检出限( LOD)为8μg/L。结果表明,本方法具有良好的选择性,可用于实际样品的检测。
채용저몽산납환원법합성료립경위13 nm 적금납미입자,병재기표면수식상곡광감태( GSH-AuNPs)。재일정적염농도범위내,곡광감태능보호금납미입자면수염유도적취집。당신경원소3( Neuroge-nin3,ngn3)다태편단존재시,재일정적염농도하,ngn3편단능구유도GSH-AuNPs발생취집,사금납미입자용액유홍색변성람색。이곡광감태수식적금납미입자위탐침,건립료쾌속검측ngn3편단적비색방법。통과우화득도적최괄실험조건위：ngn3여 GSH-AuNPs 적평형반응시간10 min,완충액pH=6.0, NaCl 농도100 mmol/L。재우화조건하,검측ngn3적선성범위위20~300μg/L,검출한( LOD)위8μg/L。결과표명,본방법구유량호적선택성,가용우실제양품적검측。
The 13 nm gold nanoparticles ( AuNPs ) were synthesized through the reduction of HAuCl4 by sodium citrate and the glutathione was assembled on the AuNPs. Under the experiment conditions, glutathione-modified AuNPs ( GSH-AuNPs ) with negative charge presented a wine red color owing to the electrostatic repulsion between nanoparticals. Upon the addition of neurogenin 3 ( ngn3 ) peptide, the aggregation of GSH-AuNPs was induced by ngn3 peptide under a certain concentration of salt. The color of AuNPs solution changed from red to blue as a function of the ngn3 peptide concentration. Thus, a rapid detection method for ngn3 peptide using GSH-AuNPs as colorimetric probe was established. The optimal experiment conditions were equilibrium time=10 min, pH=6. 0, CNaCl=100 mmol/L. Under the optimum conditions, the assay showed a linear response range of 20-300 μg/L for the peptide with a detection limit being 8 μg/L and exhibited excellent selectivity for ngn3 peptide.