防护林科技
防護林科技
방호림과기
PROTECTION FOREST SCIENCE AND TECHNOLOGY
2014年
7期
24-26,46
,共4页
栗生枝%刘立国%柴晓东
慄生枝%劉立國%柴曉東
률생지%류입국%시효동
松枯枝病%哈茨木霉%绿色木霉%POD%CAT%SOD
鬆枯枝病%哈茨木黴%綠色木黴%POD%CAT%SOD
송고지병%합자목매%록색목매%POD%CAT%SOD
Cenangium ferruginosum%Trichoderma harzianum%Trichoderma viride%POD%CAT%SOD
为探明绿色木霉和哈茨木霉的提取物对松枯枝病的抑制作用机制，通过绿色木霉（Trichoderma viride）和哈茨木霉（Trichoderma harzianum）提取物对松枯枝病病原菌（Cenangium ferruginosum Fr ．）进行平板表面涂抹法和混合培养基法抑制生长试验，观察松枯枝病病原菌生长情况，结果表明，2种木霉菌的提取物对松枯枝病病原菌均有抑制作用，且效果比较明显，抑制效果最佳的为绿色木霉，病原菌的被抑制率达53．54％，混合培养基法中哈茨木霉菌20％提取物对枯枝病原菌的抑制率高达31．67％。
為探明綠色木黴和哈茨木黴的提取物對鬆枯枝病的抑製作用機製，通過綠色木黴（Trichoderma viride）和哈茨木黴（Trichoderma harzianum）提取物對鬆枯枝病病原菌（Cenangium ferruginosum Fr ．）進行平闆錶麵塗抹法和混閤培養基法抑製生長試驗，觀察鬆枯枝病病原菌生長情況，結果錶明，2種木黴菌的提取物對鬆枯枝病病原菌均有抑製作用，且效果比較明顯，抑製效果最佳的為綠色木黴，病原菌的被抑製率達53．54％，混閤培養基法中哈茨木黴菌20％提取物對枯枝病原菌的抑製率高達31．67％。
위탐명록색목매화합자목매적제취물대송고지병적억제작용궤제，통과록색목매（Trichoderma viride）화합자목매（Trichoderma harzianum）제취물대송고지병병원균（Cenangium ferruginosum Fr ．）진행평판표면도말법화혼합배양기법억제생장시험，관찰송고지병병원균생장정황，결과표명，2충목매균적제취물대송고지병병원균균유억제작용，차효과비교명현，억제효과최가적위록색목매，병원균적피억제솔체53．54％，혼합배양기법중합자목매균20％제취물대고지병원균적억제솔고체31．67％。
In order to ascertain the inhibitory mechanism of extract from Trichoderma viride & Trichoderma harzi-anum ,the experiments to inhibit the growth for the extract of Trichoderma viride & Trichoderma harzianum on Cenangium ferruginosum by using method of plate & mix-cultivation media were conducted .Result shows that :the extracts of two kinds of Trichoderma spp .all have inhibition on Cenangium ferruginosum and have significant re-sult .T .v iride has the optimal inhibition result ,the inhibition rate of pathogens is 53 .54% ;the inhibition rate of 20%extract of Trichoderma harzianum on Cenangium ferruginosum are 31 .67% .
