医学理论与实践
醫學理論與實踐
의학이론여실천
The Journal of Medical Theory and Practice
2014年
11期
1401-1402,1413
,共3页
李雪华%刘燕青%谷彦军%刘静
李雪華%劉燕青%穀彥軍%劉靜
리설화%류연청%곡언군%류정
肺癌%血清蛋白质组学%双向凝胶电泳
肺癌%血清蛋白質組學%雙嚮凝膠電泳
폐암%혈청단백질조학%쌍향응효전영
Lung cancer%Serum proteomics%Two-dimensional polyacrylamide gel electrophoresis
目的：建立肺癌血清蛋白质双向凝胶电泳（2-DE）图谱。方法：对肺癌血清蛋白质2-DE的IPG胶条选择、上样量、聚焦参数、样品缓冲液、沉淀处理等各方面进行调整、优化。结果：获得了稳定的肺癌血清蛋白质2-DE图谱。结论：已建立的肺癌血清蛋白质2-DE图谱，可以为进一步开展肺癌的血清蛋白质组学研究奠定基础。
目的：建立肺癌血清蛋白質雙嚮凝膠電泳（2-DE）圖譜。方法：對肺癌血清蛋白質2-DE的IPG膠條選擇、上樣量、聚焦參數、樣品緩遲液、沉澱處理等各方麵進行調整、優化。結果：穫得瞭穩定的肺癌血清蛋白質2-DE圖譜。結論：已建立的肺癌血清蛋白質2-DE圖譜，可以為進一步開展肺癌的血清蛋白質組學研究奠定基礎。
목적：건립폐암혈청단백질쌍향응효전영（2-DE）도보。방법：대폐암혈청단백질2-DE적IPG효조선택、상양량、취초삼수、양품완충액、침정처리등각방면진행조정、우화。결과：획득료은정적폐암혈청단백질2-DE도보。결론：이건립적폐암혈청단백질2-DE도보，가이위진일보개전폐암적혈청단백질조학연구전정기출。
Objective :To establish the map of serum proteins for lung cancer by two-dimensional polyacrylamide gel e-lectrophoresis(2-DE) .Methods :Various conditions of serum proteins 2-DE ,such as choice of IPG strips ,quantity of loading proteins ,parameters of IEF ,buffer of proteins ,precipitation of samples ,were adjusted and optimized .Results:A steady 2-DE map of serum proteins for lung cancer was established .Conclusion:Our established 2-DE map of serum proteins for lung cancer can be effectively applied in serum proteomics of lung cancer .
