中国现代药物应用
中國現代藥物應用
중국현대약물응용
CHINESE JOURNAL OF MODERN DRUG APPLICATION
2014年
12期
18-19
,共2页
丹田胶囊%丹参酮ⅡA%高效液相色谱
丹田膠囊%丹參酮ⅡA%高效液相色譜
단전효낭%단삼동ⅡA%고효액상색보
Dantian Capsule%TanshinoneⅡA%High performance liquid chromatography
目的：测定丹田胶囊中丹参酮ⅡA含量。方法采用高效液色谱法(HPLC)测定制剂中丹参酮ⅡA的含量。选择C18柱,以甲醇-水(73：27)为流动相,流速为1.0 ml/min,检测波长为270 nm。结果丹参酮ⅡA在0.016256~0.325120μg/ml的范围内线性关系良好, Y=33.8899 X+35.5178, r=0.99994(n=5),平均回收率为102.5%, RSD为1.4%(n=6)。结论该方法简便、准确、专属性强,可用于该制剂的含量质量控制。
目的：測定丹田膠囊中丹參酮ⅡA含量。方法採用高效液色譜法(HPLC)測定製劑中丹參酮ⅡA的含量。選擇C18柱,以甲醇-水(73：27)為流動相,流速為1.0 ml/min,檢測波長為270 nm。結果丹參酮ⅡA在0.016256~0.325120μg/ml的範圍內線性關繫良好, Y=33.8899 X+35.5178, r=0.99994(n=5),平均迴收率為102.5%, RSD為1.4%(n=6)。結論該方法簡便、準確、專屬性彊,可用于該製劑的含量質量控製。
목적：측정단전효낭중단삼동ⅡA함량。방법채용고효액색보법(HPLC)측정제제중단삼동ⅡA적함량。선택C18주,이갑순-수(73：27)위류동상,류속위1.0 ml/min,검측파장위270 nm。결과단삼동ⅡA재0.016256~0.325120μg/ml적범위내선성관계량호, Y=33.8899 X+35.5178, r=0.99994(n=5),평균회수솔위102.5%, RSD위1.4%(n=6)。결론해방법간편、준학、전속성강,가용우해제제적함량질량공제。
Objective To determinate tanshinone ⅡA content in Dantian Capsule. Methods The tanshinoneⅡA content in this medicine was determined by high performance liquid chromatography(HPLC). The methanol-water(73:27)solution was used as the mobile phase, the column was C18, the flow rate was 1.0 ml/min. The detective wavelength was at 270 nm. Results Tanshinone showed a good linear relationship at the range of 0.016256~0.325120μg/ml, Y=7645.44538X=6.10968, r=0.99994(n=5). The average recovery was 102.5%with RSD of 1.4%(n=6). Conclusion The method is simple, reliable, accurate and can be applied to the quality control for this preparation.
