CAJ | 학술논문

目的：探讨miR-130a-3p对人乳腺癌MCF-7细胞功能的影响及其可能的靶向基因。方法：应用脂质体介导方法将miR-130a-3p模拟物（实验组）或对照模拟物（对照组）转染MCF-7细胞，分别采用MTT方法和细胞划痕实验检测细胞增殖和迁移能力变化，印迹法检测细胞中miR-130a-3p靶基因圆柱瘤基因（CYLD）的表达。结果：与对照组比较，实验组MCF-7细胞的增殖和迁移能力增强（P＜0.05），细胞中CYLD蛋白表达水平明显下调（P＜0.05）。结论：miR-130a-3p可能通过靶向调节CYLD蛋白表达增强人乳腺癌MCF-7细胞的增殖和迁移。
목적：탐토miR-130a-3p대인유선암MCF-7세포공능적영향급기가능적파향기인。방법：응용지질체개도방법장miR-130a-3p모의물（실험조）혹대조모의물（대조조）전염MCF-7세포，분별채용MTT방법화세포화흔실험검측세포증식화천이능력변화，인적법검측세포중miR-130a-3p파기인원주류기인（CYLD）적표체。결과：여대조조비교，실험조MCF-7세포적증식화천이능력증강（P＜0.05），세포중CYLD단백표체수평명현하조（P＜0.05）。결론：miR-130a-3p가능통과파향조절CYLD단백표체증강인유선암MCF-7세포적증식화천이。
Objective:To investigate the effect of miR-130a-3p on the function of human breast cancer cell line MCF-7, and to explore its possible target gene. Methods: miR-130a-3p mimics or its negative control (miR-130a-3p mimics-NC) was transfected into MCF-7 cells by lipofectamine. The capacities of proliferation and migration of MCF-7 cells were evaluated by MTT and Scratch assay, respectively. The expression of CYLD which was predicted as a target gene in MCF-7 cells was determined by Western blotting. Results:The capacities of proliferation and migration in MCF-7 cells transfected with miR-130a-3p mimics were signiifcantly promoted and the expression level of CYLD was down-regulated when compared with those in MCF-7 cells transfected with miR-130a-3p mimics-NC(P<0.05). Conclusion:The miR-130a-3p expression can promote the proliferation and migration of human breast cancer line MCF-7 by down-regulating the expression of CYLD.