CAJ | 학술논문

目的：对UniCel DXI 800型全自动微粒子化学发光分析仪定量检测血清黄体酮（Prog）进行性能评价。方法运用该系统检测血清Prog，并对其灵敏度、精密度、准确度及特异性等进行方法学评价。同时与Access 2免疫分析系统进行相关性比较。结果该系统检测血清 Prog 的精密度较高，变异系数小于10％，最低检测限为0．1 ng/mL，回收率范围为95．3％～104．7％，抗干扰能力各项指标均达到较高水准。与Access 2化学发光免疫系统结果比较具有很好的相关性，得到回归方程：Y＝0．84X-0．63，r＝0．978。结论该系统对定量检测血清Prog 有较高的灵敏度和高特异性，且精密度符合临床要求，与Access 2检测系统有很好的相关性，值得推广。
목적：대UniCel DXI 800형전자동미입자화학발광분석의정량검측혈청황체동（Prog）진행성능평개。방법운용해계통검측혈청Prog，병대기령민도、정밀도、준학도급특이성등진행방법학평개。동시여Access 2면역분석계통진행상관성비교。결과해계통검측혈청 Prog 적정밀도교고，변이계수소우10％，최저검측한위0．1 ng/mL，회수솔범위위95．3％～104．7％，항간우능력각항지표균체도교고수준。여Access 2화학발광면역계통결과비교구유흔호적상관성，득도회귀방정：Y＝0．84X-0．63，r＝0．978。결론해계통대정량검측혈청Prog 유교고적령민도화고특이성，차정밀도부합림상요구，여Access 2검측계통유흔호적상관성，치득추엄。
Objective To evaluate the quantitative detection of serum progesterone by UnicellDXI-800 automated chemilumines-cent analyzer.Methods To detect the serum progesterone,and evaluate the sensitivity,precision,accuracy and specificity.At the same time,the correlation with Access 2 immune system was analyzed as well.Results The precision of this system of serum pro-gesterone detection was high,with the variation coefficient less than 10%,the sensitivity of 0.1 ng/mL,and the recovery range of 95.3% to 104.7%.And the anti-jamming capability indexes reached higher level.It had good correlation compareing with the pre-vious Access 2 chemiluminescence immune system,and the regression equation obtained wasY= 0.84X-0.63,r= 0.978.Conclu-sion The system for quantitative detection of serum progesterone had high sensitivity and specificity,and its precision could consistent with clinical requirements.It has good correlation with Access 2 chemiluminescence immune system and worthy of promotion.