CAJ | 학술논문

目的：分析和探讨乙型肝炎病毒（HBV）血清标志物 ELISA 法检测条件的优化途径。方法比较洗板机洗板后用蒸馏水或干净自来水冲洗反应板与不冲洗反应板的临界值标本比例。结果洗板后用蒸馏水再次冲洗反应板数秒后、用干净自来水再次冲洗反应板与不冲洗反应板的表面抗原临界值标本比例（含临界阴性和弱阳性）分别为0．52％、2．60％和5．56％；表面抗体临界值标本比例（含临界阴性和弱阳性）分别为5．52％、8．02％和14．86％；e抗原临界值标本比例（含临界阴性和弱阳性）分别为2．82％、2．92％和3．55％。结论洗板结束后用蒸馏水或干净自来水再次冲洗反应孔边缘可降低因酶标试剂或显色剂污染反应孔边缘导致的高临界值标本比例。蒸馏水比干净自来水更适合作为反应孔边缘的冲洗液。
목적：분석화탐토을형간염병독（HBV）혈청표지물 ELISA 법검측조건적우화도경。방법비교세판궤세판후용증류수혹간정자래수충세반응판여불충세반응판적림계치표본비례。결과세판후용증류수재차충세반응판수초후、용간정자래수재차충세반응판여불충세반응판적표면항원림계치표본비례（함림계음성화약양성）분별위0．52％、2．60％화5．56％；표면항체림계치표본비례（함림계음성화약양성）분별위5．52％、8．02％화14．86％；e항원림계치표본비례（함림계음성화약양성）분별위2．82％、2．92％화3．55％。결론세판결속후용증류수혹간정자래수재차충세반응공변연가강저인매표시제혹현색제오염반응공변연도치적고림계치표본비례。증류수비간정자래수경괄합작위반응공변연적충세액。
Objective To analyze and approach the optimizing of ELISA assay for HBV serum markers.Methods To compare the critical value rate of flushing with distilled water,flushing with cleaning tap water and flushing without anything.Results The HBsAg critical value rates of flushing with distilled water,flushing with cleaning tap water and flushing without anything were 0.52%,2.60% and 5.56%.The HBsAb critical value rates of flushing with distilled water,flushing with cleaning tap water and flushing without anything were 5.52%,8.02% and 14.86%.The HBeAg critical value rate of flushing with distilled water,flush-ing with cleaning tap water and flushing without anything were 2.82%,2.92%and 3.55%.Conclusion Flushing with distilled wa-ter or flushing with cleaning tap water can reduce the high critical value rate because of enzyme or developer pollution.Flushing with distilled water exhibits more efficacy than flushing with cleaning tap water.