CAJ | 학술논문

目的：通过金标法、酶联免疫吸附测定（ELISA）法和光激化学发光（LiCA）法3种方法检测 HBsAg，评价3种检测方法在临床上的适用性。方法选取1000例标本，每一例标本都采用3种方法进行检测，筛查可疑的 HBsAg阳性标本进行复检，鉴别假阴性、假阳性。结果3种检测方法中，LiCA法最为敏感和特异，其次是 ELISA 法，金标法的灵敏度虽然略低于两者，但金标法最大的特点就是简单、快速，其灵敏度可达1 ng/mL，适合急诊或术前患者的初步筛查，能满足临床快速检测的需求。ELISA法由于操作步骤多易造成假阴性、假阳性，但是其灵敏度高、特异性强、价格低廉，更易被大众所接受，适合大批量样本检测。LiCA法虽然价格贵，但其特有的高稳定性、高准确性、易操作性能更好地反映患者体内 HBV复制状况以及抗病毒药物的疗效。结论临床应根据需求选择适宜的 HBsAg检测方法。
목적：통과금표법、매련면역흡부측정（ELISA）법화광격화학발광（LiCA）법3충방법검측 HBsAg，평개3충검측방법재림상상적괄용성。방법선취1000례표본，매일례표본도채용3충방법진행검측，사사가의적 HBsAg양성표본진행복검，감별가음성、가양성。결과3충검측방법중，LiCA법최위민감화특이，기차시 ELISA 법，금표법적령민도수연략저우량자，단금표법최대적특점취시간단、쾌속，기령민도가체1 ng/mL，괄합급진혹술전환자적초보사사，능만족림상쾌속검측적수구。ELISA법유우조작보취다역조성가음성、가양성，단시기령민도고、특이성강、개격저렴，경역피대음소접수，괄합대비량양본검측。LiCA법수연개격귀，단기특유적고은정성、고준학성、역조작성능경호지반영환자체내 HBV복제상황이급항병독약물적료효。결론림상응근거수구선택괄의적 HBsAg검측방법。
Objective To evaluate the clinical applicability of Gold Lebel,ELISA and LiCA,through detecting HBsAg by all the three methods.Methods A total of 1 000 sapmles were selected,and each sample was detected HBsAg by using three methods. Each positive sampale was detected repeatedly to identify false negative and false positive.Results In all the three methods,the most sensitive and specific method was LiCA,next was ELISA,and the last was Gold Lebel.But Gold Lebel method was more sim-ple and rapid for preliminary screening for emergency or preoperative patients with its sensibility of 1 ng/mL.ELISA had too many operating steps to bring false negative and false positive,while its high sensitivity and specificity and low price might be accepted by commonage and fit for mass simples.LiCA′s especially high stability,accuracy,easily operating could better reflex HBV replication and therapy effects of antiviral drugs in spite of its slight cost.Conclusion The suitable HBsAg detection method should be chosen according to clinical demands.