国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
12期
1612-1613,1615
,共3页
结核分枝杆菌%聚合酶链反应%抗酸染色%DNA%结核抗体
結覈分枝桿菌%聚閤酶鏈反應%抗痠染色%DNA%結覈抗體
결핵분지간균%취합매련반응%항산염색%DNA%결핵항체
Mycobacterium tuberculosis%polymerase chain reaction%acid fast staining%DNA%tuberculosis antibody
目的：探讨DNA和结核抗体（TB-Ab）联合检测在结核性胸膜炎的临床诊断意义。方法分别采用斑点金免疫渗滤法和实时荧光定量PCR法对95例结核性胸腔积液患者及45例非结核性胸腔积液患者进行TB-Ab和结核分枝杆菌DNA检测，并对结果进行分析比较。结果结核性胸膜炎组结核分枝杆菌 DNA 检测阳性率为56．8％，显著高于非结核性胸膜炎组（4．4％），差异具有统计学意义（P＜0．05）。结核性胸膜炎组TB-Ab检测阳性率为58．9％，显著高于非结核性胸膜炎组（6．6％），差异具有统计学意义（P＜0．05）。两项指标应用结核性胸膜炎组阳性率可提高为78．9％，显著高于涂片镜检以及结核菌培养单项检测阳性率，差异具有统计学意（P＜0．01）。结论结核分枝杆菌DNA 与TB-Ab 联合检测可明显提高结核性胸膜炎的阳性检出率，而且快速、简便、易操作。
目的：探討DNA和結覈抗體（TB-Ab）聯閤檢測在結覈性胸膜炎的臨床診斷意義。方法分彆採用斑點金免疫滲濾法和實時熒光定量PCR法對95例結覈性胸腔積液患者及45例非結覈性胸腔積液患者進行TB-Ab和結覈分枝桿菌DNA檢測，併對結果進行分析比較。結果結覈性胸膜炎組結覈分枝桿菌 DNA 檢測暘性率為56．8％，顯著高于非結覈性胸膜炎組（4．4％），差異具有統計學意義（P＜0．05）。結覈性胸膜炎組TB-Ab檢測暘性率為58．9％，顯著高于非結覈性胸膜炎組（6．6％），差異具有統計學意義（P＜0．05）。兩項指標應用結覈性胸膜炎組暘性率可提高為78．9％，顯著高于塗片鏡檢以及結覈菌培養單項檢測暘性率，差異具有統計學意（P＜0．01）。結論結覈分枝桿菌DNA 與TB-Ab 聯閤檢測可明顯提高結覈性胸膜炎的暘性檢齣率，而且快速、簡便、易操作。
목적：탐토DNA화결핵항체（TB-Ab）연합검측재결핵성흉막염적림상진단의의。방법분별채용반점금면역삼려법화실시형광정량PCR법대95례결핵성흉강적액환자급45례비결핵성흉강적액환자진행TB-Ab화결핵분지간균DNA검측，병대결과진행분석비교。결과결핵성흉막염조결핵분지간균 DNA 검측양성솔위56．8％，현저고우비결핵성흉막염조（4．4％），차이구유통계학의의（P＜0．05）。결핵성흉막염조TB-Ab검측양성솔위58．9％，현저고우비결핵성흉막염조（6．6％），차이구유통계학의의（P＜0．05）。량항지표응용결핵성흉막염조양성솔가제고위78．9％，현저고우도편경검이급결핵균배양단항검측양성솔，차이구유통계학의（P＜0．01）。결론결핵분지간균DNA 여TB-Ab 연합검측가명현제고결핵성흉막염적양성검출솔，이차쾌속、간편、역조작。
Objective To disscuss the significance of combined detection of DNA and tuberculosis antibody(TB-Ab)in the diag-nosis of tuberculous pleurisy.Methods Dot immunogold filtration assay and real-time fluorescence PCR method were respectively used to detect TB-Ab and DNA of 95 cases of tuberculous pleural effusion patients(tuberculosis pleurisy group)and 45 cases of pa-tients without tuberculous pleural effusion(non-tuberculous pleurisy group).Results were analyzed and compared.Results The positive rate of Mycobacterium tuberculosis DNA in tuberculosis pleurisy group was 5 6 .8%,which was higher than that of non-tu-berculous pleurisy group(4.4%),with significant difference between them(P<0.05).The positive rate of TB-Ab in tuberculosis pleurisy group was 58.9%,higher than that of non tuberculous pleurisy group (6.6%),and the difference was statistically signifi-cant (P<0.05).Combined application of DNA and TB-Ab,the positive rate of tuberculosis pleurisy group could be increased to 78.9%,significantly higher than the positive rates of individual test of smear or bacterial culture(P<0.01).Conclusion Combined detection of Mycobacterium tuberculosis DNA and TB-Ab can significantly improve the positive detection rate of tuberculous pleuri-sy,and the method is fast,simple and easy to operate.
