CAJ | 학술논문

目的：研究徐州地区汉族RhD阴性人群RHD基因多态性。方法采用常规血清学技术检测RhD抗原表型，采用抗球蛋白试验（IAT）确认结果。采用序列特异性引物-聚合酶链反应（SSP-PCR）方法检测RHD基因。结果110例RhD阴性个体中，SSP-PCR检测RHD基因结果为RHD 阳性基因携带者5例，RHD阴性基因携带者47例，RHD-CE（2-9）-D 基因携带者22例，DVI Ⅲ型基因携带者17例，弱D15基因携带者2例，DEL-1227A基因携带者17例。结论徐州地区汉族人群RhD阴性个体呈现RHD基因结构复杂的多态性，以变异体等位基因RH-CE（2-9）-D 基因、DVI Ⅲ型基因、DEL-1227A基因为主。
목적：연구서주지구한족RhD음성인군RHD기인다태성。방법채용상규혈청학기술검측RhD항원표형，채용항구단백시험（IAT）학인결과。채용서렬특이성인물-취합매련반응（SSP-PCR）방법검측RHD기인。결과110례RhD음성개체중，SSP-PCR검측RHD기인결과위RHD 양성기인휴대자5례，RHD음성기인휴대자47례，RHD-CE（2-9）-D 기인휴대자22례，DVI Ⅲ형기인휴대자17례，약D15기인휴대자2례，DEL-1227A기인휴대자17례。결론서주지구한족인군RhD음성개체정현RHD기인결구복잡적다태성，이변이체등위기인RH-CE（2-9）-D 기인、DVI Ⅲ형기인、DEL-1227A기인위주。
Objective To investigate the RHD gene polymorphism among the RhD negative Han individuals in Xuzhou.Methods The RhD antigen phenotypes were detected by routine serological techniques,and were identified with indirect antiglobulin test (IAT).The genotypes of RHD were detected by using sequence specific primer polymerase chain reaction(SSP-PCR).Results A-mong 110 RhD negative individuals,there were 5 cases carring RHD positive gene,47 cases carring RHD negative gene,22 cases carring RHD-CE(2-9)-D gene,17 cases carring DVI Ⅲ gene,2 cases carring weak D15 gene,and 17 carring DEL-1227A gene,re-spectively.Conclusion The RhD negative Han individuals present complex RHD gene polymorphism in Xuzhou region,and variant alleles such as RHD-CE(2-9)-D,DVI Ⅲ,DEL-1227A are given priority.