CAJ | 학술논문

背景：脐带间充质干细胞的培养是进行脐带间充质干细胞研究时极其重要的部分，细胞培养技术的优化对推动间充质干细胞的临床应用特别是细胞治疗至关重要。同时，脐带间充质干细胞标记及示踪技术是干细胞移植治疗的研究热点之一。目的：对国内外脐带间充质干细胞染色标记技术的研究与进展作一综述。方法：以“干细胞，间充质干细胞，脐带间充质干细胞，细胞培养，染色标记”为中文捡索词，以“Umbilical cord-derived mesenchymalstem cel s，Cel culture，Labeling methods”为英文检索词，检索维普和中国知网(CNKI)期刊全文数据库、Medline，highwire和外文生物医学期刊全文数据库(Foreign Journals Integration System)2001年1月至2013年10月有关脐带间充质干细胞的培养及标记染色文献。最终纳入35篇文献进行综述。结果与结论：脐带间充质干细胞尚未得到广泛应用，最关键的原因是脐带间充质干细胞的分离培养及染色技术不是很成熟，这些方面都值得在研究中进一步优化。虽然近几年脐带间充质干细胞标记及示踪技术有了较快的进展，但仍存在许多问题有待进一步解决。
배경：제대간충질간세포적배양시진행제대간충질간세포연구시겁기중요적부분，세포배양기술적우화대추동간충질간세포적림상응용특별시세포치료지관중요。동시，제대간충질간세포표기급시종기술시간세포이식치료적연구열점지일。목적：대국내외제대간충질간세포염색표기기술적연구여진전작일종술。방법：이“간세포，간충질간세포，제대간충질간세포，세포배양，염색표기”위중문검색사，이“Umbilical cord-derived mesenchymalstem cel s，Cel culture，Labeling methods”위영문검색사，검색유보화중국지망(CNKI)기간전문수거고、Medline，highwire화외문생물의학기간전문수거고(Foreign Journals Integration System)2001년1월지2013년10월유관제대간충질간세포적배양급표기염색문헌。최종납입35편문헌진행종술。결과여결론：제대간충질간세포상미득도엄범응용，최관건적원인시제대간충질간세포적분리배양급염색기술불시흔성숙，저사방면도치득재연구중진일보우화。수연근궤년제대간충질간세포표기급시종기술유료교쾌적진전，단잉존재허다문제유대진일보해결。
The culture of umbilical cord-derived mesenchymal stem cells is extremely important for studies on umbilical cord mesenchymal stem cells. Optimization of cellculture technology is crucial for clinical application of mesenchymal stem cells and even celltherapy. Meanwhile, the labeling and tracer technique of umbilical cord-derived mesenchymal stem cells is a hotspot in stem celltransplantation. OBJECTIVE:To review the research and development of the cellmarkers and tracer methods of umbilical cord-derived mesenchymal stem cells. METHODS:A computer-based search of VIP, CNKI, Medline, Highwire and Foreign Journals Integration System databases was performed for articles concerning culture and labeling of umbilical cord-derived mesenchymal stem cells published from January 2001 to October 2013. The keywords were“stem cells, mesenchymal stem cells, umbilical cord-derived mesenchymalstem cells, cellculture, labeling methods”in Chinese and English, respectively. Final y, 35 articles were included in result analysis. RESULTS AND CONCLUSION:Umbilical cord-derived mesenchymal stem cells have not yet been widely used, mainly because of the immature isolation, culture and staining techniques of umbilical cord-derived mesenchymal stem cells. These techniques are worthy of further optimization studies. Although in recent years, cellmarkers and tracer technology of umbilical cord-derived mesenchymal stem cell s have made great progress, there are stil many problems need to be solved.