CAJ | 학술논문

背景：与来源于中胚层间充质的躯干四肢骨不同，颌骨来源于颅神经嵴外胚间充质，具有独特的膜内成骨机制。核心结合因子Cbfα1是骨分化的关键性转录因子，但其p56亚型在颌骨组织中的作用尚不明确。目的：研究Cbfα1/p56亚型在大鼠颌骨来源骨髓间充质干细胞中的表达及意义。方法：原代培养大鼠颌骨和髂骨来源的骨髓间充质干细胞，采用ELISA法检测骨髓间充质干细胞中的碱性磷酸酶活性，荧光定量PCR法检测骨髓间充质干细胞中Cbfα1/p56和p57亚型的mRNA相对表达量。结果与结论：成功培养出大鼠颌骨和髂骨来源的骨髓间充质干细胞，颌骨来源骨髓间充质干细胞的增殖能力及碱性磷酸酶活性明显高于髂骨；在培养第6天，颌骨来源骨髓间充质干细胞的Cbfα1/p56亚型mRNA相对表达量高于髂骨来源骨髓间充质干细胞(P<0.05)，而Cbfα1/p57亚型在各培养时间点的mRNA 相对表达量两组间差异均无显著性意义(P>0.05)。结果提示Cbfα1/p56亚型对颌骨来源骨髓间充质干细胞的早期成骨分化具有重要意义。
배경：여래원우중배층간충질적구간사지골불동，합골래원우로신경척외배간충질，구유독특적막내성골궤제。핵심결합인자Cbfα1시골분화적관건성전록인자，단기p56아형재합골조직중적작용상불명학。목적：연구Cbfα1/p56아형재대서합골래원골수간충질간세포중적표체급의의。방법：원대배양대서합골화가골래원적골수간충질간세포，채용ELISA법검측골수간충질간세포중적감성린산매활성，형광정량PCR법검측골수간충질간세포중Cbfα1/p56화p57아형적mRNA상대표체량。결과여결론：성공배양출대서합골화가골래원적골수간충질간세포，합골래원골수간충질간세포적증식능력급감성린산매활성명현고우가골；재배양제6천，합골래원골수간충질간세포적Cbfα1/p56아형mRNA상대표체량고우가골래원골수간충질간세포(P<0.05)，이Cbfα1/p57아형재각배양시간점적mRNA 상대표체량량조간차이균무현저성의의(P>0.05)。결과제시Cbfα1/p56아형대합골래원골수간충질간세포적조기성골분화구유중요의의。
BACKGROUND:Unlike the ilium derived from the paraxial mesoderm, the mandible from cranial neural crest has a unique mechanism. Core binding factorα1 (Cbfα1) is a key transcription factor for skeletogenic process. However, the role of Cbfα1/p56 subtype in mandible tissue is yet not clear. OBJECTIVE:To research the expression of Cbfα1/p56 subtype in bone marrow mesenchymal cells from rat mandible in vitro. METHODS:Bone marrow mesenchymal stem cells from rat mandible and ilium were in vitro isolated and purified by primary culture. The characteristics of bone marrow mesenchymal cells were compared through the methods of enzyme linked immunosorbent assay and real-time PCR, including growth curve, alkaline phosphatase activity and relative mRNA expression of Cbfα1 subtypes. RESULTS AND CONCLUSION:Bone marrow mesenchymal cells from rat mandible and ilium were successful y obtained. Bone marrow mesenchymal cells from the mandible proliferated more rapidly, alkaline phosphatase activity of which was higher than iliac cells. The relative mRNA expression of Cbfα1/p56 subtype in bone marrow mesenchymal cells from the mandible was more than that in iliac cells at 6 days of culture (P<0.05), while the expression of Cbfα1/p57 in each time showed no statistical significance (P>0.05). The results showed that Cbfα1/p56 is very significant in the early osteogenic differentiation of bone marrow mesenchymal cells from the mandible.