CAJ | 학술논문

背景：间充质干细胞移植后的归巢能力关系到细胞移植的疗效，研究其趋化和迁移的调控将有助于提高间充质干细胞临床应用的价值。目的：观察Cdc42在人脐带间充质干细胞定向迁移中的作用。方法：首先以组织块法分离培养人脐带间充质干细胞，与炎性因子肿瘤坏死因子α、白细胞介素1β、转化生长因子β共培养后 Western 检测 Cdc42表达变化。化学合成 Cdc42的干扰 RNA，转染细胞后分别采用Transwel 和Matrigel胶观察细胞迁移和黏附能力。应用Western检测Cdc42下游靶分子ERK1/2的变化。结果与结论：与炎性因子共培养后的人脐带间充质干细胞中Cdc42表达明显增加，接近无因子对照组的2倍水平。siRNA下调Cdc42的表达能够显著抑制人脐带间充质干细胞的迁移和黏附，且其下游信号分子ERK1/2的表达以及磷酸化水平均相应受到抑制。提示体外培养环境下Cdc42参与了人脐带间充质干细胞的趋化过程。
배경：간충질간세포이식후적귀소능력관계도세포이식적료효，연구기추화화천이적조공장유조우제고간충질간세포림상응용적개치。목적：관찰Cdc42재인제대간충질간세포정향천이중적작용。방법：수선이조직괴법분리배양인제대간충질간세포，여염성인자종류배사인자α、백세포개소1β、전화생장인자β공배양후 Western 검측 Cdc42표체변화。화학합성 Cdc42적간우 RNA，전염세포후분별채용Transwel 화Matrigel효관찰세포천이화점부능력。응용Western검측Cdc42하유파분자ERK1/2적변화。결과여결론：여염성인자공배양후적인제대간충질간세포중Cdc42표체명현증가，접근무인자대조조적2배수평。siRNA하조Cdc42적표체능구현저억제인제대간충질간세포적천이화점부，차기하유신호분자ERK1/2적표체이급린산화수평균상응수도억제。제시체외배양배경하Cdc42삼여료인제대간충질간세포적추화과정。
BACKGROUND:The homing ability of mesenchymal stem cells is closely associated with the effects of celltransplantation. Clarifying the mechanism of chemotaxis and migration wil contribute to enhance the clinical application of mesenchymal stem cells. OBJECTIVE:To investigate the effect of Cdc42 in the homing of human umbilical cord mesenchymal stem cells. METHODS:First, mesenchymal stem cells were isolated from human umbilical cord, and co-cultured with tumor necrosis factorα, interleukin-1β, and transforming growth factorβ. Western blot assay was used to test the level of Cdc42. Besides, Cdc42 siRNA was synthesized by chemical method to transfect the cells, and cellmigration and adhesion were measured by Transwel and Matrigel separately. Meanwhile, the activity of signal molecule, extracellular regulated protein kinase 1/2, was evaluated by western blot. RESULTS AND CONCLUSION:The results indicated that the inflammation factors induced the highly expression of Cdc42 in human umbilical cord mesenchymal stem cells, almost double level to controls. siRNA notably inhibited the migration and adhesion of human umbilical cord mesenchymal stem cells through Cdc42 down-regulation, and the extracellular regulated protein kinase 1/2 and phosphorylation form were also decreased simultaneously. In a word, we speculate Cdc42 plays a role in the chemotaxis of human umbilical cord mesenchymal stem cells in vitro.