CAJ | 학술논문

目的：考察替莫唑胺壳聚糖微球的制备工艺，并对C6脑胶质瘤细胞的抑制作用进行了研究。方法：采用紫外分光光度法测定微球中替莫唑胺的含量，以壳聚糖为载体，采用乳化-化学交联法制备替莫唑胺壳聚糖微球；以替莫唑胺包封率为评价指标进行微球制备工艺的筛选；显微镜观察微球形态学特征；SRB法考察替莫唑胺壳聚糖微球体外抗肿瘤作用。结果：所制得微球形态圆整，粒径分布均匀，平均粒径为（217±1．19） nm，包封率为27．6±0．37％，对C6胶质瘤细胞具有显著的抑制作用。结论：本法制备替莫唑胺壳聚糖微球的工艺稳定，且对C6胶质瘤细胞具有显著的抑制作用。
목적：고찰체막서알각취당미구적제비공예，병대C6뇌효질류세포적억제작용진행료연구。방법：채용자외분광광도법측정미구중체막서알적함량，이각취당위재체，채용유화-화학교련법제비체막서알각취당미구；이체막서알포봉솔위평개지표진행미구제비공예적사선；현미경관찰미구형태학특정；SRB법고찰체막서알각취당미구체외항종류작용。결과：소제득미구형태원정，립경분포균균，평균립경위（217±1．19） nm，포봉솔위27．6±0．37％，대C6효질류세포구유현저적억제작용。결론：본법제비체막서알각취당미구적공예은정，차대C6효질류세포구유현저적억제작용。
Objective:Temozolomide chitosan microspheres was prepared and investigated the preparation technolo -gy,then studied the inhibitory effect of C 6 glioma cells.Methods:The amount of temozolomide which in nanospheres was analyzed by ultraviolet spectrophotometry .Temozolomide chitosan microspheres was prepared by emulsion-chemical crosslink technique ,with using chitosan as a carrier .The preparation process of microspheres was optimized by temozolomide encapsu-lation efficiency as index .The particles size and morphological characteristics of chitosan microspheres were observed by mi -croscopic.And SRB method was used to study the anti-tumor effect of temozolomide chitosan microspheres on C 6 glioma cells.Results:The temozolomide chitosan microspheres were spherical in shape and even in particle size .The mean diame-ter was(217 ±1.19) nm and the average entrapment efficifency of temozolomide chitosan microspheres was 27.6 ±0. 37%.And have significant inhibitory effects on C 6 glioma cells than free temozolomide .Conclusion:The temozolomide chi-tosan microspheres was tability and have sustained release effect .And have a significantly inhibited on C 6 glioma cells.