CAJ | 학술논문

目的：分析FKBP12和FKBP52在肝细胞癌（hepatocellular carcinoma，HCC）组织中的表达，并探讨其与HCC临床病理因素的关系。方法应用免疫组化SP法检测FKBP12和FKBP52在60例HCC组织、60例癌旁组织、10例肝血管瘤组织和10例正常肝组织中的表达水平，并结合临床病理因素分析其临床意义。结果在HCC组织、癌旁组织、肝血管瘤组织和正常肝组织中FKBP12的阳性表达率分别为66.7%、30.0%、20.0%和40.0%，FKBP12在HCC组织中的表达明显高于癌旁组织、肝血管瘤组织和正常肝组织中的表达（P均约0.05）。在HCC组织、癌旁组织、肝血管瘤组织和正常肝组织中FKBP52的阳性表达率分别为63.3%、26.7%、40.0%和20.0%，FKBP52在HCC组织中的表达明显高于癌旁组织和正常肝组织中的表达（P均约0.05），与肝血管瘤组织中的表达比较差异无统计学意义（P跃0.05）。FKBP12和KBP52在HCC组织中的表达比较差异无统计学意义（P跃0.05）。FKBP12在HCC组织中的表达水平与患者年龄、性别、合并肝硬化和乙肝病毒感染无明显关系（P均跃0.05），而与肿瘤直径、病理分级和血清中AFP的水平有关（P均约0.05）。FKBP52在HCC组织中的表达水平与患者年龄、性别、肿瘤直径、合并肝硬化和血清中AFP的水平无明显关系（P均跃0.05），而与病理分级、乙肝病毒感染有关（P均约0.05）。结论 FKBP12和FKBP52的高表达与HCC的发生、发展密切相关。FKBP12可能参与HCC癌细胞的增殖以及分化过程，并且可能与HCC患者血清中AFP水平的升高有关；FKBP52可能参与HCC的分化过程，并且可能与乙肝病毒感染密切相关。
목적：분석FKBP12화FKBP52재간세포암（hepatocellular carcinoma，HCC）조직중적표체，병탐토기여HCC림상병리인소적관계。방법응용면역조화SP법검측FKBP12화FKBP52재60례HCC조직、60례암방조직、10례간혈관류조직화10례정상간조직중적표체수평，병결합림상병리인소분석기림상의의。결과재HCC조직、암방조직、간혈관류조직화정상간조직중FKBP12적양성표체솔분별위66.7%、30.0%、20.0%화40.0%，FKBP12재HCC조직중적표체명현고우암방조직、간혈관류조직화정상간조직중적표체（P균약0.05）。재HCC조직、암방조직、간혈관류조직화정상간조직중FKBP52적양성표체솔분별위63.3%、26.7%、40.0%화20.0%，FKBP52재HCC조직중적표체명현고우암방조직화정상간조직중적표체（P균약0.05），여간혈관류조직중적표체비교차이무통계학의의（P약0.05）。FKBP12화KBP52재HCC조직중적표체비교차이무통계학의의（P약0.05）。FKBP12재HCC조직중적표체수평여환자년령、성별、합병간경화화을간병독감염무명현관계（P균약0.05），이여종류직경、병리분급화혈청중AFP적수평유관（P균약0.05）。FKBP52재HCC조직중적표체수평여환자년령、성별、종류직경、합병간경화화혈청중AFP적수평무명현관계（P균약0.05），이여병리분급、을간병독감염유관（P균약0.05）。결론 FKBP12화FKBP52적고표체여HCC적발생、발전밀절상관。FKBP12가능삼여HCC암세포적증식이급분화과정，병차가능여HCC환자혈청중AFP수평적승고유관；FKBP52가능삼여HCC적분화과정，병차가능여을간병독감염밀절상관。
Objective To analyze the expression of FKBP12 and FKBP52 in hepatocellular carcinoma(HCC)and explore its rela-tionship with clinicopathological factors. Methods Expression of FKBP12 and FKBP52 was measured in 60 samples of HCC tissue, 60 samples of normal adjacent tissue,10 samples of hepatic hemangioma and 10 cases of normal liver tissue.Expression was analyzed using the immunohistochemical streptavidin-peroxidase method and correlated with clinicopathological factors. Results Expression of FKBP12 was detected in 66.7%of HCC samples,a rate significantly higher than the 30.0%of adjacent tissues,20.0%of liver hemangioma and 40.0% of normal liver tissues(P<0.05).The rate of FKBP52 expression was significantly higher in HCC tissues(63.3%)than in adjacent tissues(26.7%)and normal liver tissues(20.0%),but similar to the rate in liver hemangioma(40.0%).Rates of FKBP12 and FKBP52 expression were similar in HCC tissues.There was no significant relationship between FKBP12 expression in HCC tissue and patient age,gender,cirrhosis,or hepatitis B virus infection,but expression was related to tumor size,pathology grade,and serum AFP level.There was no significant relationship between FKBP52 expression in HCC tissue and age,gender,tumor size,cirrhosis, or serum AFP level(P>0.05),but expression was related to pathology grade and hepatitis B virus infection(P<0.05). Conclusion Expression of FKBP12 and FKBP52 correlate closely with incidence of HCC.FKBP12 may be involved in the proliferation and differentiation of tumor cells,and it may contribute to elevated serum AFP levels in liver cancer patients.FKBP52 may contribute to the differentiation of hepatocellular carcinoma and may participate in cellular processes in liver infected by hepatitis B virus.