CAJ | 학술논문

建立了RP-HPLC法测定紫油厚朴药材中和厚朴酚及厚朴酚的含量．采用依利特ODS2 C18（200 mm×4．6 mm，5μm）色谱柱，以甲醇∶水＝75∶25（ V/V）为流动相，检测波长294 nm，流速1．0 mL/min，柱温25℃．结果得到厚朴酚的平均回收率为98．10％，RSD为0．42％，和厚朴酚的平均回收率为99．86％，RSD为0．86％．该方法重复性好、精密度高、线性关系良好，快速、准确；为紫油厚朴药材品质评价、品种选育及含量控制提供了科学依据．
건립료RP-HPLC법측정자유후박약재중화후박분급후박분적함량．채용의리특ODS2 C18（200 mm×4．6 mm，5μm）색보주，이갑순∶수＝75∶25（ V/V）위류동상，검측파장294 nm，류속1．0 mL/min，주온25℃．결과득도후박분적평균회수솔위98．10％，RSD위0．42％，화후박분적평균회수솔위99．86％，RSD위0．86％．해방법중복성호、정밀도고、선성관계량호，쾌속、준학；위자유후박약재품질평개、품충선육급함량공제제공료과학의거．
The HPLC is used to determine the content of purple Magnolia officinalis medicinal herbs and Magnolol and honokiol,content.ODS2C18 (200 mm x 4.6 mm,5μm) chromatographic column is a dopt-ed,and methanol ∶water=75∶25(V/V) is taken as mobile phase.Detection wavelength of 294 nm,ve-locity:1.0 ml/min,column temperature 25℃.The results of Magnolol , the average recovery was 98.10%, RSD=0.42%.Honokiol the average recovery was 99.86%,RSD=0.86%.The method has good repeatabili-ty, high precision ,good linearity ,rapidity and high accuracy .and provides a scientific basis for Magnolia officinalis medicinal materials quality evaluation ,variety breeding and content control .