CAJ | 학술논문

连翘是最广泛使用的传统中药之一，具有抗菌、抗病毒、抗炎和抗氧化活性等重要的药理作用，而连翘苷是连翘的主要生物活性成分。为了从连翘叶中快速简便地提取、分离和纯化得到纯度较高的连翘苷，试验采用无水乙醇和体积浓度85%乙醇回流提取，大孔树脂柱层析和石油醚萃取的方法分离，重结晶法进行纯化，经不同组合试验以确定最佳制备工艺。通过 HPLC 测定比较所得连翘苷的含量、纯度，最终确定了以无水乙醇回流提取，石油醚萃取分离，重结晶纯化的工艺流程，通过该方法可以得到纯度达90%的连翘苷。
련교시최엄범사용적전통중약지일，구유항균、항병독、항염화항양화활성등중요적약리작용，이련교감시련교적주요생물활성성분。위료종련교협중쾌속간편지제취、분리화순화득도순도교고적련교감，시험채용무수을순화체적농도85%을순회류제취，대공수지주층석화석유미췌취적방법분리，중결정법진행순화，경불동조합시험이학정최가제비공예。통과 HPLC 측정비교소득련교감적함량、순도，최종학정료이무수을순회류제취，석유미췌취분리，중결정순화적공예류정，통과해방법가이득도순도체90%적련교감。
Forsythia suspensa is one of most widely used traditional Chinese medicines, and possesses important biolog-ical activities, including antibacterial, antiviral, anti-inflammatory and antioxidant activities. Forsythin is the main bioac-tive component of forsythia suspensa. This paper aimed to obtain highly purified forsythin from forsythia suspensa leaves quickly and easily, and to study its pharmacological effects in future. The study designed a series of methods, extraction methods containing anhydrous ethanol and 85% ethanol extract, separation methods adopting macroporous resin column chromatography and petroleum ether extraction, purification method employing recrystallization. Through HPLC deter-mined the content and purity of forsythin, a better method was founded which included anhydrous ethanol extraction, sepa-ration of petroleum ether and recrystallization. At last, a certain amount of 90% purity forsythin had been extracted by the method.