生物医学工程学进展
生物醫學工程學進展
생물의학공정학진전
PROGRESS IN BIOMEDICAL ENGINEERING
2014年
2期
84-88
,共5页
细菌%分类鉴定%自动化鉴定
細菌%分類鑒定%自動化鑒定
세균%분류감정%자동화감정
bacteria%classification and identification%automated identification
细菌的分类鉴定与细菌的研究和应用有着同等重要的地位。准确地鉴定细菌类别,根据其菌种种类不同,分别针对肠杆菌、阳性球菌、非发酵菌等菌种设计不同药敏板条的抗生素组合,可以指导临床应用用药。目前,细菌分类鉴定方法主要包括表型鉴定法和分子遗传学鉴定法两大类,针对这两大类鉴定方法,汇总其生理生化分类特征,简述各方法采用的关键技术,针对各技术讨论其优缺点。同时详细研究了表型鉴定法下数值分类法中自动化鉴定涉及的算法,结合目前细菌鉴定方法,对细菌分类鉴定的发展趋势进行了总结和展望。
細菌的分類鑒定與細菌的研究和應用有著同等重要的地位。準確地鑒定細菌類彆,根據其菌種種類不同,分彆針對腸桿菌、暘性毬菌、非髮酵菌等菌種設計不同藥敏闆條的抗生素組閤,可以指導臨床應用用藥。目前,細菌分類鑒定方法主要包括錶型鑒定法和分子遺傳學鑒定法兩大類,針對這兩大類鑒定方法,彙總其生理生化分類特徵,簡述各方法採用的關鍵技術,針對各技術討論其優缺點。同時詳細研究瞭錶型鑒定法下數值分類法中自動化鑒定涉及的算法,結閤目前細菌鑒定方法,對細菌分類鑒定的髮展趨勢進行瞭總結和展望。
세균적분류감정여세균적연구화응용유착동등중요적지위。준학지감정세균유별,근거기균충충류불동,분별침대장간균、양성구균、비발효균등균충설계불동약민판조적항생소조합,가이지도림상응용용약。목전,세균분류감정방법주요포괄표형감정법화분자유전학감정법량대류,침대저량대류감정방법,회총기생리생화분류특정,간술각방법채용적관건기술,침대각기술토론기우결점。동시상세연구료표형감정법하수치분류법중자동화감정섭급적산법,결합목전세균감정방법,대세균분류감정적발전추세진행료총결화전망。
The classification and identification of bacteria are equally important compared to its research and application. After bacteria categories are accurately identified,different antibiotic susceptibility lath antibiotic combination,which are specific to bacteria species,such as enteric bacilli,positive coccus,non-fermenter and other bacteria respectively,can be designed to guide clinic drugs application. Currently,methods of bacteria classi-fication and identification mainly include phenotypic and molecular genetic identification. According to these two methods for the classification and identification of bacteria,their physiological and biochemical characteristics were summarized. The key techniques and corresponding merits and defects were systematically elaborated and dis-cussed. Simultaneously,relevant algorithms in numerical taxonomy and automated identification of the phenotypic identification method were studied. Combined with the current method of bacterial identification,this article sum-marized and prospected the development trend of bacteria identification in the future.