CAJ | 학술논문

目的：检测食管鳞癌患者不同临床分期癌组织及癌旁组织中MicroRNA let-7e表达水平的差异。方法：应用实时荧光定量RT-PCR Taqman MGB探针法，检测60例食管癌癌组织及癌旁组织中MicroRNA let-7e表达水平。以U6 snRNA为内参，进行荧光定量PCR,统计分析表达水平的差异。结果：MicroRNA let-7e的表达水平在癌组织比癌旁组织高，病理分期I期与III期相比，差异有统计学意义（P＜0.05）。结论：MicroRNA let-7e在食管鳞癌中表达水平明显高于癌旁组织，可能用于食管鳞癌临床分期诊断的重要标记。
목적：검측식관린암환자불동림상분기암조직급암방조직중MicroRNA let-7e표체수평적차이。방법：응용실시형광정량RT-PCR Taqman MGB탐침법，검측60례식관암암조직급암방조직중MicroRNA let-7e표체수평。이U6 snRNA위내삼，진행형광정량PCR,통계분석표체수평적차이。결과：MicroRNA let-7e적표체수평재암조직비암방조직고，병리분기I기여III기상비，차이유통계학의의（P＜0.05）。결론：MicroRNA let-7e재식관린암중표체수평명현고우암방조직，가능용우식관린암림상분기진단적중요표기。
Purpose: To investigate the expression levels of MicroRNA let-7e in esophageal squamous cell cancer patients and to compare cancer tissues with adjacent normal lung tissues in different clinical stages. Method: The MicroR-NA let-7e expression levels in cancer tissues and adjacent normal lung tissues were detected by quantitative real-time re-verse transcription-PCR from 60 esophageal squamous cell cancer patients who were divided into three groups, with U6 RNA as an actin. Results: The expression levels of MicroRNA let-7e in cancer tissues were significantly higher than in adjacent normal esophageal tissues, and had statistical significance in different stages of the tumors: characteristics, lymph metastasis, differentiation, and the stage of the tumors. Conclusions: The study suggested that microRNA let-7e was sig-nificantly up-expressed in esophageal squmaos cell cancer and should be a useful biomarker for the diagnosis of clinical stages of esophageal cancer patients.