CAJ | 학술논문

目的：探讨山楂总黄酮对雄性Wistar糖尿病（ DM）大鼠睾丸组织的保护作用及其PCNA表达影响。方法：Wistar雄性大鼠40只，随机分为正常对照组、糖尿病模型组、胰岛素治疗组和山楂叶总黄酮治疗组（ HLF组）。，每组10只，尾静脉注射1％链尿佐菌素（STZ）溶液诱导糖尿病模型。 HLF组给予山楂叶总黄酮200mg/（ kg· d）定时灌胃给药。取一侧睾丸组织甲醛固定，用于制作免疫荧光切片，激光共聚焦显微镜观察。结果：2型糖尿病模型制备成功， HLF治疗后大鼠血糖显著低于模型组（p＜0.01），大鼠体重和睾丸质量明显增加，曲细精管直径接近于正常，HLF组大鼠睾丸组织PCNA含量显著高于对照组（ p＜0.01）。结论：山楂叶总黄酮能改善糖尿病大鼠睾丸损伤提升睾丸组织中PCNA的含量表达，对睾丸组织具有明显保护作用。
목적：탐토산사총황동대웅성Wistar당뇨병（ DM）대서고환조직적보호작용급기PCNA표체영향。방법：Wistar웅성대서40지，수궤분위정상대조조、당뇨병모형조、이도소치료조화산사협총황동치료조（ HLF조）。，매조10지，미정맥주사1％련뇨좌균소（STZ）용액유도당뇨병모형。 HLF조급여산사협총황동200mg/（ kg· d）정시관위급약。취일측고환조직갑철고정，용우제작면역형광절편，격광공취초현미경관찰。결과：2형당뇨병모형제비성공， HLF치료후대서혈당현저저우모형조（p＜0.01），대서체중화고환질량명현증가，곡세정관직경접근우정상，HLF조대서고환조직PCNA함량현저고우대조조（ p＜0.01）。결론：산사협총황동능개선당뇨병대서고환손상제승고환조직중PCNA적함량표체，대고환조직구유명현보호작용。
Objective:To observe the changes of PCNA expression in diabetes mellitus rats'testis and explore the effect of HLF on diabetes mellitus rats'procreation function .Methods:40 male Wistar rats were divided into 4 groups ( Control group , Diabetes mellitus model group , Insulinized group and HLF group ) and there were ten in each group .Diabetic rat model was induced by a vena caudalis injection of streptozotocin (30mg/kg).Rats in HLF group received HLF for 200mg /kg.d by gavage.One testis was taken and fixed in formaldehyde to make immunofluorescence section and pathological section .Results:Type 2 diabetes mellitus models were success-fully constructed after treated by HLF.Their blood sugar concentration was significantly lower than model group (p<0.01),and their weight as well as testis increased significantly .The diameter of convoluted seminiferous tubule was close to the normal level .The PC-NA expression of HLF group was significantly higher than that of Model group (p<0.01).Conclusion:Improving testis injury in dia-betic rats and promoting the expression of PCNA significantly , HLF could protect testis tissue .