首都医科大学学报
首都醫科大學學報
수도의과대학학보
JOURNAL OF CAPITAL UNIVERSITY OF MEDICAL SCIENCES
2014年
4期
501-506
,共6页
魏华%景红%吴江%张东航
魏華%景紅%吳江%張東航
위화%경홍%오강%장동항
胃癌%葡萄糖调节蛋白78%葡萄糖调节蛋白94%生物标志物%预后
胃癌%葡萄糖調節蛋白78%葡萄糖調節蛋白94%生物標誌物%預後
위암%포도당조절단백78%포도당조절단백94%생물표지물%예후
gastric cancer%glucose-regulated protein 78%glucose-regulated protein 94%biomarker%prognosis
目的：确定葡萄糖调节蛋白78（glucose-regulated protein 78，GRP78）和葡萄糖调节蛋白94（glucose-regulated protein 94， GRP94）与胃癌（ gastric cancer ，GC）临床病理特征及预后的相关性。方法应用Western blotting和免疫组织化学方法检测GC和切缘组织中GRP78和GRP94的表达；Kaplan-Meier或卡方检验GRP78、GRP94和临床病理资料与GC术后整体生存期相关性。结果 GRP78和GRP94蛋白主要表达于胃癌细胞的胞质及胞膜，其阳性表达与淋巴结转移、TNM分期呈负相关，与分化程度正相关（P＜0．05）；GRP78、GRP94高表达及低表达GC患者术后平均生存时间分别为（23．51±2．32）个月和（37．54±3．24）个月、（21．31±1．46）个月和（40．02±2．19）个月，5年整体生存率分别为21．23％和41．42％、20．73％和43．86％（log-rank test，P＜0．01），TNM分期、GRP78和GRP94是影响GC预后的独立因素。结论 GRP78和GRP94高表达可能是GC预后不良的分子标志物之一。
目的：確定葡萄糖調節蛋白78（glucose-regulated protein 78，GRP78）和葡萄糖調節蛋白94（glucose-regulated protein 94， GRP94）與胃癌（ gastric cancer ，GC）臨床病理特徵及預後的相關性。方法應用Western blotting和免疫組織化學方法檢測GC和切緣組織中GRP78和GRP94的錶達；Kaplan-Meier或卡方檢驗GRP78、GRP94和臨床病理資料與GC術後整體生存期相關性。結果 GRP78和GRP94蛋白主要錶達于胃癌細胞的胞質及胞膜，其暘性錶達與淋巴結轉移、TNM分期呈負相關，與分化程度正相關（P＜0．05）；GRP78、GRP94高錶達及低錶達GC患者術後平均生存時間分彆為（23．51±2．32）箇月和（37．54±3．24）箇月、（21．31±1．46）箇月和（40．02±2．19）箇月，5年整體生存率分彆為21．23％和41．42％、20．73％和43．86％（log-rank test，P＜0．01），TNM分期、GRP78和GRP94是影響GC預後的獨立因素。結論 GRP78和GRP94高錶達可能是GC預後不良的分子標誌物之一。
목적：학정포도당조절단백78（glucose-regulated protein 78，GRP78）화포도당조절단백94（glucose-regulated protein 94， GRP94）여위암（ gastric cancer ，GC）림상병리특정급예후적상관성。방법응용Western blotting화면역조직화학방법검측GC화절연조직중GRP78화GRP94적표체；Kaplan-Meier혹잡방검험GRP78、GRP94화림상병리자료여GC술후정체생존기상관성。결과 GRP78화GRP94단백주요표체우위암세포적포질급포막，기양성표체여림파결전이、TNM분기정부상관，여분화정도정상관（P＜0．05）；GRP78、GRP94고표체급저표체GC환자술후평균생존시간분별위（23．51±2．32）개월화（37．54±3．24）개월、（21．31±1．46）개월화（40．02±2．19）개월，5년정체생존솔분별위21．23％화41．42％、20．73％화43．86％（log-rank test，P＜0．01），TNM분기、GRP78화GRP94시영향GC예후적독립인소。결론 GRP78화GRP94고표체가능시GC예후불량적분자표지물지일。
Objective This study aimed to determine the correlation of glucose-regulated protein 78 ( GRP78 ) and glucose-regulated protein 94(GRP94) expression with clinical features and its prognostic value in gastric cancer (GC).Methods Western blotting and immunohistochemistry ( IHC) were used to estimate the GRP 78 and GRP94 expression in GC and distal margin of resected GC tissues .The association of GRP78 and GRP94 protein expression with clinical features and GC prognosis was determined by Kaplan-Meier curves. Results GRP78 and GRP94 immunostaining dots located predominantly in the cytoplasm and plasma membrane .Both GRP78 and GRP94 were positively correlated with differentiation, and negatively with lymph node metastasis and TNM stage (P<0.05).The mean survival time of GC patients with high and low expression of GRP 78 and GRP94 were 23.51±2.32 and (37.54±3.24)months, 21.31± 1.46 and (40.02±2.19)months, respectively, and the overall 5 year survival rate 21.23% and 41.42%, 20.73% and 43.86%, respectively(log-rank test, P<0.01).TNM stage and GRP78 and GRP94 were independent prognostic factors of GC .Conclusion High protein expression of GRP78 and GRP94 may correlate with poor prognosis of GC .
