CAJ | 학술논문

目的：研究豚鼠耳蜗外毛细胞的细胞膜电导和机械负荷对细胞电致运动的动力学特性的影响。方法使用分离自豚鼠耳蜗第四回的外毛细胞，采用全细胞电压钳和微室电极技术。使用以光电二极管为基础的测量系统来测量电致运动。结果外毛细胞的膜电导可以同时影响电致运动的灵敏度和运动的非线性，而机械负载可以显著影响电致运动的幅度、不对称和频率响应。结论外毛细胞的膜电导和机械负荷可以影响外毛细胞的电致运动，从而影响耳蜗放大器功能。
목적：연구돈서이와외모세포적세포막전도화궤계부하대세포전치운동적동역학특성적영향。방법사용분리자돈서이와제사회적외모세포，채용전세포전압겸화미실전겁기술。사용이광전이겁관위기출적측량계통래측량전치운동。결과외모세포적막전도가이동시영향전치운동적령민도화운동적비선성，이궤계부재가이현저영향전치운동적폭도、불대칭화빈솔향응。결론외모세포적막전도화궤계부하가이영향외모세포적전치운동，종이영향이와방대기공능。
Objective To examine the influence of membrane conductances and mechanical load on the dynamic characteristics of outer hair cell ( OHC) motility.Methods All the experiments were performed on OHCs isolated from the 4th turns of guinea pig cochleae, using whole-cell voltage-clamp and microchamber techniques .Motility was measured using a photodiode-based measurement system.Results Membrane conductances influence both sensitivity and nonlinearity of motility , and that mechanical load significantly affects magnitude , asymmetry and frequency response of motility .Conclusion Membrane conductances and mechanical load influence OHC motility, thereby, the cochlear amplifier .