CAJ | 학술논문

目的：探讨调节性T细胞（regulatory T cells，Tregs）在急性肝衰竭外周血及肝脏中表达频数的变化。方法选取10名急性肝衰竭患者及5名健康对照者，应用流式细胞术检测各组患者外周血中调节性T细胞表达频数的变化。将Balb/C小鼠采用数字表法随机分成急性肝衰竭1d、3d、7d组和正常对照组。模型组小鼠腹腔注射四氯化碳（carbon tetrachloride，CCL4）矿物油1次，建立急性肝衰竭模型，对照组注射等量0．9％氯化钠注射液。生化法测定血清ALT、AST浓度，HE染色观察肝组织病理形态变化；流式细胞术分析外周血和肝组织中浸润的Tregs变化。结果流式分析结果显示，与正常对照组相比，急性肝衰竭患者外周血Tregs表达下降（3．23±1．87 vs 6．08±1．45，P＜0．05）。与对照组相比，急性肝衰竭1 d、3 d和7 d组小鼠外周血中Tregs 比例CD25＋Foxp3＋/CD4＋与对照组相比呈下降趋势，而肝组织中Tregs 比例CD25＋Foxp3＋/CD4＋呈升高趋势。结论急性肝衰竭时患者和小鼠外周血中调节性T细胞呈下降趋势，而小鼠肝中浸润的CD4＋CD25＋Foxp3＋调节性T细胞呈升高趋势，Tregs可能与急性肝衰竭的发生有一定相关性。
목적：탐토조절성T세포（regulatory T cells，Tregs）재급성간쇠갈외주혈급간장중표체빈수적변화。방법선취10명급성간쇠갈환자급5명건강대조자，응용류식세포술검측각조환자외주혈중조절성T세포표체빈수적변화。장Balb/C소서채용수자표법수궤분성급성간쇠갈1d、3d、7d조화정상대조조。모형조소서복강주사사록화탄（carbon tetrachloride，CCL4）광물유1차，건립급성간쇠갈모형，대조조주사등량0．9％록화납주사액。생화법측정혈청ALT、AST농도，HE염색관찰간조직병리형태변화；류식세포술분석외주혈화간조직중침윤적Tregs변화。결과류식분석결과현시，여정상대조조상비，급성간쇠갈환자외주혈Tregs표체하강（3．23±1．87 vs 6．08±1．45，P＜0．05）。여대조조상비，급성간쇠갈1 d、3 d화7 d조소서외주혈중Tregs 비례CD25＋Foxp3＋/CD4＋여대조조상비정하강추세，이간조직중Tregs 비례CD25＋Foxp3＋/CD4＋정승고추세。결론급성간쇠갈시환자화소서외주혈중조절성T세포정하강추세，이소서간중침윤적CD4＋CD25＋Foxp3＋조절성T세포정승고추세，Tregs가능여급성간쇠갈적발생유일정상관성。
Objective To investigate the changes of CD 4+CD25+Foxp3+regulatory T cells ( Tregs ) in the peripheral blood and liver during acute liver failure .Methods By using flow cytometry , the peripheral blood in 10 cases of acute liver failure patients and five healthy controls were analyzed .The Balb/C mice were randomly divided into acute liver failure 1 d, 3 d, 7 d group and normal control group.The model of acute liver failure was induced by intraperitoneal injection of carbon tetrachloride (CCL4), at the same time, the control group was injected with an equal amount of 0.9% sodium chloride.The serum was collected for detecting ALT and AST .The pathological changes of liver tissue were observed by HE staining .The changes of peripheral blood and liver tissue infiltration of Tregs were analyzed by flow cytometry .Results Compared with normal persons , the Tregs frequency in peripheral blood of acute liver failure decreased, (3.23±1.87) vs (6.08±1.45), P<0.05.Certainly, in animal experiment, the proportion of Tregs in peripheral blood of ALF, CD25+Foxp3+/CD4+, decreased compared to controls〔(3.80±1.59)%, (5.23±1.32)%, (3.34±1.05)%vs (5.93±0.25)%;P>0.05, P>0.05, P>0.05〕.However, the flow cytometric analysis showed that Tregs frequency , CD25+Foxp3+/CD4+, was higher in mouse liver of acute liver failure 1 d, 3 d and 7 d than the control group〔(3.73±1.73)%, (5.93±0.60 )%, (11.16±4.65)% vs (3.33±0.99)%;P>0.05, P>0.05, P<0.05〕.Conclusion In acute liver failure, intrahepatic CD4+CD25+Foxp3+regulatory T cells showed an increasing trend , while decreased in peripheral blood in mice and patients .Tregs may have a certain correlation with the incidence of acute liver failure .