CAJ | 학술논문

目的：研究不同的标本保存方法和时间对血清总二氧化碳（ total carbon dioxide ，TCO2）检测结果的影响。方法保证TCO2检测系统稳定性符合实验室质量目标的前提下，利用高值QC（quality control）、低值QC、混合血清3种样本，分别检测室温敞口、室温封口、4℃敞口、4℃封口时不同时间点TCO2的浓度，计算TCO2浓度随时间的变化率。结果高值QC、低值QC、混合血清在室温敞口保存75 min时，TCO2结果分别下降了9．65％、13．42％、7．74％；4℃敞口保存75 min时，TCO2结果分别下降了8．96％、8．60％、3．94％；在室温封口保存90 min时，TCO2结果分别下降了4．53％、9．43％、3．94％；在4℃封口保存2 h，TCO2结果仅分别下降了3．44％、3．56％、2．19％。结论随着标本保存时间的延长血清TCO2浓度不断下降，并且不同的保存方法下降的速率不同。封口保存TCO2结果下降低于敞口状态，保存温度越低TCO2结果下降越慢。
목적：연구불동적표본보존방법화시간대혈청총이양화탄（ total carbon dioxide ，TCO2）검측결과적영향。방법보증TCO2검측계통은정성부합실험실질량목표적전제하，이용고치QC（quality control）、저치QC、혼합혈청3충양본，분별검측실온창구、실온봉구、4℃창구、4℃봉구시불동시간점TCO2적농도，계산TCO2농도수시간적변화솔。결과고치QC、저치QC、혼합혈청재실온창구보존75 min시，TCO2결과분별하강료9．65％、13．42％、7．74％；4℃창구보존75 min시，TCO2결과분별하강료8．96％、8．60％、3．94％；재실온봉구보존90 min시，TCO2결과분별하강료4．53％、9．43％、3．94％；재4℃봉구보존2 h，TCO2결과부분별하강료3．44％、3．56％、2．19％。결론수착표본보존시간적연장혈청TCO2농도불단하강，병차불동적보존방법하강적속솔불동。봉구보존TCO2결과하강저우창구상태，보존온도월저TCO2결과하강월만。
Objective To study the effect of sample storage method and time on the test results of serum total carbon dioxide . Methods Under the condition that total carbon dioxide ( TCO2 ) measurement system can guarantee the stability of TCO 2 results.TCO2 of two quality controls and a mixed serum was measured promptly at different time points after storage under various conditions :open samples at room temperature(25±2)℃, sealed samples at room temperature , open samples at 4 ℃, and sealed samples at 4 ℃.Results High level quality control(QC), low level QC and mixed serum samples decreased by 9.65%, 13.42%and 7.74%, respectively, at 75 min when samples were stored at room temperature and kept open .The same samples decreased by 4.53, 9.43 and 3.94% at 90 min when stored at room temperature and kept seal .The samples decreased by 8.96, 8.60 and 3.94% at 75 min when stored at 4 ℃ and kept open, and decreased by 3.44%, 3.56%and 2.19% at 2 h when stored at 4 ℃ and kept sealed.Conclusion TCO2 decreased over time and decreased at different rate according to storage condition .TCO2 of sealed samples decreased much slower than open samples .The colder samples stored , the slower TCO 2 decreased .