CAJ | 학술논문

研究莪术油（Zedoary Turmeric Oil, ZTO）对体外培养的人胃腺癌SGC-7901细胞形态变化的影响。不同浓度的ZTO作用人胃腺癌SGC-7901细胞24 h后，通过普通光学显微镜、荧光显微镜、透射电镜观察细胞形态变化情况。光学显微镜和荧光显微镜下可见未加药物组细胞呈梭形，胞浆丰富，核质均匀，核仁呈现黄色荧光，加药组细胞体积变小，胞浆浓缩、胞膜有起泡脱落现象，胞膜崩解，部分细胞核破碎或消失，细胞核呈现绿色或亮黄色荧光。透射电镜下可见未加药组细胞呈圆形，细胞核较大，胞浆较多，细胞器丰富，加药组细胞积明显变小，核固缩，微绒毛消失，部分坏死细胞的胞核溶解，细胞器结构崩解消失。ZTO作用人胃腺癌SGC-7901细胞24 h能致见未加药组细胞胞浆较多，细胞器丰富，加药组细胞体积明显变小，坏死细胞的胞使细胞发生明显的形态改变，且具有剂量依赖性。
연구아술유（Zedoary Turmeric Oil, ZTO）대체외배양적인위선암SGC-7901세포형태변화적영향。불동농도적ZTO작용인위선암SGC-7901세포24 h후，통과보통광학현미경、형광현미경、투사전경관찰세포형태변화정황。광학현미경화형광현미경하가견미가약물조세포정사형，포장봉부，핵질균균，핵인정현황색형광，가약조세포체적변소，포장농축、포막유기포탈락현상，포막붕해，부분세포핵파쇄혹소실，세포핵정현록색혹량황색형광。투사전경하가견미가약조세포정원형，세포핵교대，포장교다，세포기봉부，가약조세포적명현변소，핵고축，미융모소실，부분배사세포적포핵용해，세포기결구붕해소실。ZTO작용인위선암SGC-7901세포24 h능치견미가약조세포포장교다，세포기봉부，가약조세포체적명현변소，배사세포적포사세포발생명현적형태개변，차구유제량의뢰성。
To study the effects of ZTO on the morphological changes of human gastric cancer SGC-7901 cell in vitro. Different concentration of ZTO effect on human gastric cancer SGC-7901 cell for 24 h,using light microscope, fluorescence microscope,transmission electron microscope to observe the changes in cell morphology. Results:Optical microscope and fluorescence microscope result showed without adding drug group cells were spindle shaped, with abundant cytoplasm, nucleolar uniform,nuclear was yellow fluorescence staining,cell was smaller,cytoplasmic condensation,cell membrane with bubbling off phenomenon,cell membrane disintegration, some nucleus were broken or disappeared,the nucleus was green or bright yellow fluorescence. Transmission electron microscopy showed no drug group cells were round, large nucleus,cytoplasm,abundant organelles,dosing group cells accumulate significantly smaller,nuclear pyknosis,microvilli disappeared, cell necrosis,organelle structure disintegration disappear. ZTO effect on human gastric cancer SGC-7901 cell for 24 h can cause obvious morphological changes of cells, in a dose-dependent manner.