CAJ | 학술논문

目的：本研究旨在探讨AKT-Foxo3a信号通路在基质金属蛋白酶-20（matrix metalloprotein-ase-20，MMP-20）基因表达中的作用。方法：分别通过免疫组化技术、实时定量RT-PCR技术以及双荧光素酶报告基因检测系统分析AKT-Foxo3 a信号通路对MMP-20启动子转录活性的影响。结果：免疫组化染色结果显示，AKT及磷酸化Foxo3 a（P-Foxo3 a）均在分泌期成釉细胞核中呈阳性表达；RT-PCR检测结果显示，AKT阻断剂可显著抑制Foxo3a 诱导的MMP-20基因的表达（P"0．05）；双荧光素酶分析结果显示，AKT阻断剂亦可显著抑制Foxo3a调控 MMP-20基因启动子的转录活性（P "0．05）。结论：转录因子 Foxo3a 可能通过AKT-Foxo3 a信号通路调控MMP-20基因的表达，从而参与釉质发育过程。
목적：본연구지재탐토AKT-Foxo3a신호통로재기질금속단백매-20（matrix metalloprotein-ase-20，MMP-20）기인표체중적작용。방법：분별통과면역조화기술、실시정량RT-PCR기술이급쌍형광소매보고기인검측계통분석AKT-Foxo3 a신호통로대MMP-20계동자전록활성적영향。결과：면역조화염색결과현시，AKT급린산화Foxo3 a（P-Foxo3 a）균재분비기성유세포핵중정양성표체；RT-PCR검측결과현시，AKT조단제가현저억제Foxo3a 유도적MMP-20기인적표체（P"0．05）；쌍형광소매분석결과현시，AKT조단제역가현저억제Foxo3a조공 MMP-20기인계동자적전록활성（P "0．05）。결론：전록인자 Foxo3a 가능통과AKT-Foxo3 a신호통로조공MMP-20기인적표체，종이삼여유질발육과정。
AIM:To investigate the role of AKT-Foxo3a signaling pathway in matrix metalloproteinase-20 (MMP-20)gene expression in mouse ameloblasts.METHODS:AKT-Foxo3a and MMP-20 expression in mouse ameloblasts were examined by immunohistochemistry.Effects of AKT-Foxo3a signaling pathway on MMP-20 promoter were investigated by real-time quantitative RT-PCR and dual luciferase reporter gene assay system.RESULTS:Im-munohistochemical staining results showed that AKT and phosphorylated Foxo3a (P-Foxo3a)were both strongly ex-pressed in the nuclei of secretory ameloblasts.By RT-PCR,it was found that AKT inhibitor reduced the Foxo3a in-duced MMP-20 gene expression (P<0.05).Dual luciferase assay showed that AKT inhibitor decreased MMP-20 pro-moter activity (P<0.05).CONCLUSION:Foxo3a regulates MMP-20 gene expression through AKT-Foxo3a signa-ling pathway in mouse ameloblasts.