CAJ | 학술논문

建立果酱和罐头中诱惑红的高效液相色谱测定方法。样品中的诱惑红色素经提取后在酸性条件下被聚酰胺粉吸附，在碱性条件下解吸附，以甲醇和乙酸铵溶液为流动相，等度洗脱，紫外检测器检测，波长254nm，以相对保留时间定性，色谱峰面积定量。浓度与峰面积线性关系良好（r=0.9995），回收率83.0%～105.0%，相对标准偏差小于20%，最低检出浓度为2.5mg/kg；该方法操作简便、灵敏、准确，适用于果酱和罐头中诱惑红的检测。
건립과장화관두중유혹홍적고효액상색보측정방법。양품중적유혹홍색소경제취후재산성조건하피취선알분흡부，재감성조건하해흡부，이갑순화을산안용액위류동상，등도세탈，자외검측기검측，파장254nm，이상대보류시간정성，색보봉면적정량。농도여봉면적선성관계량호（r=0.9995），회수솔83.0%～105.0%，상대표준편차소우20%，최저검출농도위2.5mg/kg；해방법조작간편、령민、준학，괄용우과장화관두중유혹홍적검측。
This paper aimed to establish the method for detecting allura red in jam and canned food by high performance liquid chromatography (HPLC). Allura Red was absorbed by polyamide powder after extraction under acidic conditions,and desorbed in alkaline conditions. Methanol and ammonium acetate was used as the mobile phase for isocratic elution, C18 column for separation and UV detector for detection. With wavelength 254nm, the relative retention time was qualitative and chromatographic peak area was quantitative. The linear relationship was good (r=0.9995),with a recovery rate of 83.0% to 105.0%,relative standard deviation under 20%. The lowest detectable concentration was 2.5mg/kg. The method is simple, sensitive and accurate for detection of allura red.