中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2014年
7期
968-971
,共4页
瘦素%肥胖%IFN-γ%IL-4
瘦素%肥胖%IFN-γ%IL-4
수소%비반%IFN-γ%IL-4
Leptin%Asthma%Interferon-gamma%Interleukin-4
目的：探讨肥胖哮喘儿童血清瘦素水平对Th1／Th2细胞平衡的影响，及其与临床表现的相关性。方法：选择56例中、重度哮喘儿童，根据体重指数（ BMI）将哮喘儿童分为肥胖组和非肥胖组，每组各28例，另外选择28例肥胖健康对照组以及28例正常体重健康对照组儿童；采用酶联免疫吸附测定方法检测外周血瘦素、IFN-γ和IL-4的水平，采用哮喘症状评分法评估哮喘严重程度，分析其与瘦素、IFN-γ、IL-4水平的相关性。结果：哮喘组患儿的急性期血清瘦素、IFN-γ水平均高于临床缓解期、单纯肥胖组与正常体重对照组（ P＜0畅01）；哮喘组临床缓解期与单纯肥胖组及对照组比较，血清瘦素、IFN-γ水平差异均有统计学意义（ P＜0畅05）。肥胖哮喘组的血清IL-4水平低于非肥胖哮喘组（ P＜0畅01）；肥胖哮喘组儿童症状评分高于非肥胖哮喘组（ P＜0畅01）；肥胖哮喘组儿童第1秒时间肺活量占预计值的百分率（ FEV1％）低于非肥胖哮喘组（ P＜0畅01）。肥胖哮喘组瘦素水平与IFN-γ水平正相关（ P＜0畅01），与IL-4水平负相关性（ P＜0畅01）。结论：瘦素与肥胖以及非肥胖儿童的哮喘急性发作均有关；肥胖哮喘儿童体内瘦素水平升高更明显，Th1／Th2细胞平衡向Th1偏移，IFN-γ分泌增加，临床症状更严重。
目的：探討肥胖哮喘兒童血清瘦素水平對Th1／Th2細胞平衡的影響，及其與臨床錶現的相關性。方法：選擇56例中、重度哮喘兒童，根據體重指數（ BMI）將哮喘兒童分為肥胖組和非肥胖組，每組各28例，另外選擇28例肥胖健康對照組以及28例正常體重健康對照組兒童；採用酶聯免疫吸附測定方法檢測外週血瘦素、IFN-γ和IL-4的水平，採用哮喘癥狀評分法評估哮喘嚴重程度，分析其與瘦素、IFN-γ、IL-4水平的相關性。結果：哮喘組患兒的急性期血清瘦素、IFN-γ水平均高于臨床緩解期、單純肥胖組與正常體重對照組（ P＜0暢01）；哮喘組臨床緩解期與單純肥胖組及對照組比較，血清瘦素、IFN-γ水平差異均有統計學意義（ P＜0暢05）。肥胖哮喘組的血清IL-4水平低于非肥胖哮喘組（ P＜0暢01）；肥胖哮喘組兒童癥狀評分高于非肥胖哮喘組（ P＜0暢01）；肥胖哮喘組兒童第1秒時間肺活量佔預計值的百分率（ FEV1％）低于非肥胖哮喘組（ P＜0暢01）。肥胖哮喘組瘦素水平與IFN-γ水平正相關（ P＜0暢01），與IL-4水平負相關性（ P＜0暢01）。結論：瘦素與肥胖以及非肥胖兒童的哮喘急性髮作均有關；肥胖哮喘兒童體內瘦素水平升高更明顯，Th1／Th2細胞平衡嚮Th1偏移，IFN-γ分泌增加，臨床癥狀更嚴重。
목적：탐토비반효천인동혈청수소수평대Th1／Th2세포평형적영향，급기여림상표현적상관성。방법：선택56례중、중도효천인동，근거체중지수（ BMI）장효천인동분위비반조화비비반조，매조각28례，령외선택28례비반건강대조조이급28례정상체중건강대조조인동；채용매련면역흡부측정방법검측외주혈수소、IFN-γ화IL-4적수평，채용효천증상평분법평고효천엄중정도，분석기여수소、IFN-γ、IL-4수평적상관성。결과：효천조환인적급성기혈청수소、IFN-γ수평균고우림상완해기、단순비반조여정상체중대조조（ P＜0창01）；효천조림상완해기여단순비반조급대조조비교，혈청수소、IFN-γ수평차이균유통계학의의（ P＜0창05）。비반효천조적혈청IL-4수평저우비비반효천조（ P＜0창01）；비반효천조인동증상평분고우비비반효천조（ P＜0창01）；비반효천조인동제1초시간폐활량점예계치적백분솔（ FEV1％）저우비비반효천조（ P＜0창01）。비반효천조수소수평여IFN-γ수평정상관（ P＜0창01），여IL-4수평부상관성（ P＜0창01）。결론：수소여비반이급비비반인동적효천급성발작균유관；비반효천인동체내수소수평승고경명현，Th1／Th2세포평형향Th1편이，IFN-γ분비증가，림상증상경엄중。
Objective:To investigate the levels of serum leptin and their effect on Th 1/Th2 balance and the association between serum leptin levels and clinical manifestations in obese children with asthma.Methods: All 56 children with physician-diagnosed moderate-to-severe persistent asthma and 56 healthy controls were included.The children with asthma and without asthma were divided into two groups,obese (n=28) and non-obese (n=28) by body mass index,respectively.Asthma severity was assessed by the asthma symptom score.Peripheral blood samples were collected to determine the levels of leptin , IFN-gamma and IL-4.The results were correlated with the parameters studied.Results:Serum leptin levels were significantly higher in the obese asthma group than in the non -obese asthma group ,as well as being significantly higher in the children with asthma than in the controls ( P<0.01 ) , whereas IFN-gamma levels were significantly higher and IL-4 levels were significantly lower in the obese asthma group than in the non-obese asthma group( P<0.01 ).In addition, the obese asthma group showed higher asthma symptom scores and significantly lower FEV 1 (% of predicted) than did the non-obese asthma group(P<0.01).There was a significantly positive correlation between leptin ,IFN-gamma and IL-4 levels only in the obese asthma group ( P<0.01 ).Conclusion:Leptin is involved in the pathogenesis of asthma in obese and non-obese children ,and its effect is more pronounced in the former.In the presence of high leptin levels , only obese children with asthma exhibited Th1 polarization,with higher IFN-gamma levels and greater asthma severity.
