中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2014年
7期
963-967
,共5页
刘瑛%任云青%薛丽萍%李晓颜%宋彬妤
劉瑛%任雲青%薛麗萍%李曉顏%宋彬妤
류영%임운청%설려평%리효안%송빈여
胸腺基质淋巴细胞生成素(TSLP)%核转录因子-κB p65(NF-κB p65)%子宫腺肌病
胸腺基質淋巴細胞生成素(TSLP)%覈轉錄因子-κB p65(NF-κB p65)%子宮腺肌病
흉선기질림파세포생성소(TSLP)%핵전록인자-κB p65(NF-κB p65)%자궁선기병
TSLP%NF-κB p65%Adenomyosis
目的：探讨TSLP和NF-κB在子宫腺肌病发病机制中的作用。方法：采用免疫组织化学SABC法检测35例子宫腺肌病患者异位、在位内膜及20例子宫肌瘤患者子宫内膜腺上皮细胞和间质细胞中TSLP和NF-κB p65的表达，并对两者表达量进行相关性分析；采用ELISA双抗体夹心法检测子宫腺肌病患者血清TSLP的水平，子宫肌瘤患者和健康体检者各20例作为对照。结果：子宫腺肌病患者异位、在位子宫内膜腺上皮和间质细胞中TSLP和NF-κB p65均阳性表达，且表达量明显高于对照组（子宫肌瘤组）（ P＜0.01），异位内膜腺上皮和间质细胞中TSLP和NF-κB p65表达量高于在位内膜（ P＜0.01），异位内膜腺上皮和间质细胞中TSLP和NF-κB p65的表达呈正相关，子宫腺肌病患者血清中TSLP的水平高于子宫肌瘤患者（ P＜0.05）和健康体检者（ P＜0.05）。结论：子宫腺肌病患者异位和在位子宫内膜腺上皮和间质细胞TSLP高表达，血清中水平升高，异位内膜TSLP的表达与NF-κB相关。
目的：探討TSLP和NF-κB在子宮腺肌病髮病機製中的作用。方法：採用免疫組織化學SABC法檢測35例子宮腺肌病患者異位、在位內膜及20例子宮肌瘤患者子宮內膜腺上皮細胞和間質細胞中TSLP和NF-κB p65的錶達，併對兩者錶達量進行相關性分析；採用ELISA雙抗體夾心法檢測子宮腺肌病患者血清TSLP的水平，子宮肌瘤患者和健康體檢者各20例作為對照。結果：子宮腺肌病患者異位、在位子宮內膜腺上皮和間質細胞中TSLP和NF-κB p65均暘性錶達，且錶達量明顯高于對照組（子宮肌瘤組）（ P＜0.01），異位內膜腺上皮和間質細胞中TSLP和NF-κB p65錶達量高于在位內膜（ P＜0.01），異位內膜腺上皮和間質細胞中TSLP和NF-κB p65的錶達呈正相關，子宮腺肌病患者血清中TSLP的水平高于子宮肌瘤患者（ P＜0.05）和健康體檢者（ P＜0.05）。結論：子宮腺肌病患者異位和在位子宮內膜腺上皮和間質細胞TSLP高錶達，血清中水平升高，異位內膜TSLP的錶達與NF-κB相關。
목적：탐토TSLP화NF-κB재자궁선기병발병궤제중적작용。방법：채용면역조직화학SABC법검측35례자궁선기병환자이위、재위내막급20례자궁기류환자자궁내막선상피세포화간질세포중TSLP화NF-κB p65적표체，병대량자표체량진행상관성분석；채용ELISA쌍항체협심법검측자궁선기병환자혈청TSLP적수평，자궁기류환자화건강체검자각20례작위대조。결과：자궁선기병환자이위、재위자궁내막선상피화간질세포중TSLP화NF-κB p65균양성표체，차표체량명현고우대조조（자궁기류조）（ P＜0.01），이위내막선상피화간질세포중TSLP화NF-κB p65표체량고우재위내막（ P＜0.01），이위내막선상피화간질세포중TSLP화NF-κB p65적표체정정상관，자궁선기병환자혈청중TSLP적수평고우자궁기류환자（ P＜0.05）화건강체검자（ P＜0.05）。결론：자궁선기병환자이위화재위자궁내막선상피화간질세포TSLP고표체，혈청중수평승고，이위내막TSLP적표체여NF-κB상관。
Objective:To investigate the role of TSLP and NF-κB in the pathogenesis of adenomyosis.Methods:Immunohisto-chemistry was employed to detect the expression of TSLP and NF-κB p65 in the endometrial glandular cell and stromal cell of 35 adeno-myosis patients and 20 hysteromyoma patients as control , and analyze the correlation of the two proteins.The concentration of TSLP was measured by specific ELISA in the serum of healthy subjects besides of the patients with adenomyosis and hysteromyoma .Results:TSLP and NF-κB p65 were both positively expressd in the glandular cells and stromal cells of ectopic and eutopic endometrium of patients with adenomyosis , their expression was significantly higher than that of the control group ( P<0.01 ) , and the expression of TSLP and NF-κB p65 in ectopic endometrium was significantly higher than that in eutopic endometrium ( P<0.01 ).There was positive correlation between TSLP and NF-κB p65 in ectopic endometrium.The concentration of TSLP was higher in the serum of patients with adenomyosis.Conclusion:The TSLP is highly expressed in endometrial glandular and stromal cells of patients with adenomyosis , and there is higher level of TSLP in serum , and there is correlation between the expression of TSLP and NF-κB in ectopic endometrium.
