中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2014年
7期
942-945,949
,共5页
付冰冰%孙晓鹏%赵阴环
付冰冰%孫曉鵬%趙陰環
부빙빙%손효붕%조음배
红斑狼疮,系统性%重组HMBG1%自噬%LC3
紅斑狼瘡,繫統性%重組HMBG1%自噬%LC3
홍반랑창,계통성%중조HMBG1%자서%LC3
Systemic lupus erythematosus%Recombinant human high mobility group box 1 protein%Autophagy%LC3
目的：探讨重组人高迁移率族蛋白B1（High mobility group box1，HMGB1）刺激伴肾脏损害和不伴肾脏损害的系统性红斑狼疮患者外周血单个核细胞后自噬相关蛋白---微管相关蛋白1轻链3（LC3）的两个亚型LC3Ⅱ／Ⅰ的表达情况，以及重组人HMGB1刺激对SLE患者外周血单个核细胞增殖的影响。方法：Western blot比较1μg／ml 重组人HMGB1蛋白刺激伴肾脏损害与不伴肾脏损害的系统性红斑狼疮患者外周血单个核细胞0、6、24、48 h后自噬相关蛋白LC3Ⅱ／Ⅰ的表达情况。 CCK-8法检测1μg／ml HMGB1刺激72 h对SLE患者外周血单个核细胞增殖的影响。应用SPSS17.0进行数据分析。结果：Western blot结果显示：HMGB1时间依赖性地引起SLE患者外周血单个核细胞中自噬相关蛋白LC3Ⅱ／Ⅰ表达上调（ P＜0.05）。伴肾脏损害组与不伴肾脏损害组相比，自噬水平的增加更为明显。1μg／ml HMGB1明显抑制SLE患者PBMC的增殖（ P＜0.001）。结论：HMGB1可以使SLE尤其是伴肾脏损害患者外周血单个核细胞发生细胞自噬，参与SLE甚至狼疮肾炎的发病。提示针对HMGB1的单克隆抗体或细胞自噬调节剂可能成为SLE新的治疗靶点。
目的：探討重組人高遷移率族蛋白B1（High mobility group box1，HMGB1）刺激伴腎髒損害和不伴腎髒損害的繫統性紅斑狼瘡患者外週血單箇覈細胞後自噬相關蛋白---微管相關蛋白1輕鏈3（LC3）的兩箇亞型LC3Ⅱ／Ⅰ的錶達情況，以及重組人HMGB1刺激對SLE患者外週血單箇覈細胞增殖的影響。方法：Western blot比較1μg／ml 重組人HMGB1蛋白刺激伴腎髒損害與不伴腎髒損害的繫統性紅斑狼瘡患者外週血單箇覈細胞0、6、24、48 h後自噬相關蛋白LC3Ⅱ／Ⅰ的錶達情況。 CCK-8法檢測1μg／ml HMGB1刺激72 h對SLE患者外週血單箇覈細胞增殖的影響。應用SPSS17.0進行數據分析。結果：Western blot結果顯示：HMGB1時間依賴性地引起SLE患者外週血單箇覈細胞中自噬相關蛋白LC3Ⅱ／Ⅰ錶達上調（ P＜0.05）。伴腎髒損害組與不伴腎髒損害組相比，自噬水平的增加更為明顯。1μg／ml HMGB1明顯抑製SLE患者PBMC的增殖（ P＜0.001）。結論：HMGB1可以使SLE尤其是伴腎髒損害患者外週血單箇覈細胞髮生細胞自噬，參與SLE甚至狼瘡腎炎的髮病。提示針對HMGB1的單剋隆抗體或細胞自噬調節劑可能成為SLE新的治療靶點。
목적：탐토중조인고천이솔족단백B1（High mobility group box1，HMGB1）자격반신장손해화불반신장손해적계통성홍반랑창환자외주혈단개핵세포후자서상관단백---미관상관단백1경련3（LC3）적량개아형LC3Ⅱ／Ⅰ적표체정황，이급중조인HMGB1자격대SLE환자외주혈단개핵세포증식적영향。방법：Western blot비교1μg／ml 중조인HMGB1단백자격반신장손해여불반신장손해적계통성홍반랑창환자외주혈단개핵세포0、6、24、48 h후자서상관단백LC3Ⅱ／Ⅰ적표체정황。 CCK-8법검측1μg／ml HMGB1자격72 h대SLE환자외주혈단개핵세포증식적영향。응용SPSS17.0진행수거분석。결과：Western blot결과현시：HMGB1시간의뢰성지인기SLE환자외주혈단개핵세포중자서상관단백LC3Ⅱ／Ⅰ표체상조（ P＜0.05）。반신장손해조여불반신장손해조상비，자서수평적증가경위명현。1μg／ml HMGB1명현억제SLE환자PBMC적증식（ P＜0.001）。결론：HMGB1가이사SLE우기시반신장손해환자외주혈단개핵세포발생세포자서，삼여SLE심지랑창신염적발병。제시침대HMGB1적단극륭항체혹세포자서조절제가능성위SLE신적치료파점。
Objective:To stimulate the PBMCs of systemic lupus erythematosus patients with recombinant human high mobility group box1 (HMGB1) protein,observe the effect of HMGB1 on the expression of LC3Ⅱ/Ⅰ protein in active lupus nephritis and inactive lupus nephritis patients.Evaluate the effect of recombinant human high mobility group box 1 ( HMGB1 ) on the proliferation of PBMCs in patients with SLE.Methods:Western blot was used to detect the expression of LC 3II/I protein in PBMCs of active lupus ne-phritis and inactive lupus nephritis patients after stimulated by 1 μg/ml HMGB1 for 0,6,24 and 48 h.CCK-8 assay was used to detect the effects of PBMCs proliferation in patients with SLE after 1 μg/ml HMGB1 stimulation 72 hours.The statistical software SPSS17.0 was used to analyzed the results.Results: Western bolt showed an increasing expression of LC 3Ⅱ/Ⅰ protein in SLE patients after stimulated by HMGB1 ( P<0.05 ) , and this effect was time dependent.Compared with inactive lupus nephritis group , the increasing level of autophagy in active lupus nephritis group was more obviously.1 μg/ml HMGB1 could inhibit the proliferation of PBMCs in patients of SLE significantly ( P<0.001 ).Conclusion: HMGB1 may promote autophagy in SLE especially patients with active lupus nephritis and involved in the pathogenesis of SLE and lupus nephritis.Monoclonal antibodies targeting to HMGB 1 or modulators of mam-malian autophagy may provide new way for the treatment of SLE especially LN.
