CAJ | 학술논문

目的：探索一种新的过继免疫治疗细胞体外培养的方法。方法：从正常人外周血分离单个核细胞（ PBMCs ），经过CD40激发型单抗5C11、细胞因子IFN-α、IL-7、IL-2（CD40激发组）诱导并扩增，光镜下观察其细胞形态及计数，进行动态增殖观察，并在第9天收集细胞，对其淋巴细胞亚群、NKT细胞和调节性T细胞（ Treg）的比例进行分析，同时与常规CIK细胞（ CD3激发组）进行比较性分析。结果：两组培养细胞中CD4＋／CD8＋细胞的比例无明显差异；但CD3激发组中的Treg数量和比例远高于CD40激发组，而CD3＋CD56＋的NKT细胞低于CD40激发组，同时，CD40激发组出现了一群单核-NK-DC（ Mo-NK-DC）样细胞。结论：本实验所建立的新型过继免疫治疗细胞培养方法能提高具有肿瘤杀伤能力的NKT细胞的比例，同时显著减少Treg的数量，有望为肿瘤细胞过继免疫治疗开拓新的方案。
목적：탐색일충신적과계면역치료세포체외배양적방법。방법：종정상인외주혈분리단개핵세포（ PBMCs ），경과CD40격발형단항5C11、세포인자IFN-α、IL-7、IL-2（CD40격발조）유도병확증，광경하관찰기세포형태급계수，진행동태증식관찰，병재제9천수집세포，대기림파세포아군、NKT세포화조절성T세포（ Treg）적비례진행분석，동시여상규CIK세포（ CD3격발조）진행비교성분석。결과：량조배양세포중CD4＋／CD8＋세포적비례무명현차이；단CD3격발조중적Treg수량화비례원고우CD40격발조，이CD3＋CD56＋적NKT세포저우CD40격발조，동시，CD40격발조출현료일군단핵-NK-DC（ Mo-NK-DC）양세포。결론：본실험소건립적신형과계면역치료세포배양방법능제고구유종류살상능력적NKT세포적비례，동시현저감소Treg적수량，유망위종류세포과계면역치료개탁신적방안。
Objective:To explore a new method of the cultivation of adoptive immunotherapy cells.Methods: Mononuclear cells was isoplated by density gradient centrifugation and then proliferated by using CD 40-agonist monoclonal antibody 5C11、cytokine of IFN-αand IL-7(CD40 group)in vitro.During the culturing procedure ,the cell morphology was obersved by optical microscope.The percentage of T-lymphocytes, NK-T cells, Treg cells and the cell proliferation, which were compared with CIK group CD3mAb activated,was detected on the 9th day.Results:There was no significant difference of CD 4+/CD8+T cells percentage between the two groups.But the Treg cells percentage of CIK group was far higher than that of CD 40 group,while the percentage of CD3+CD56+NK-T cells was lower than that of CD 40 group.And a group of Mo-NK-DC cells were observed in the CD 40 group.Conclusion: The new method of adoptive immunity therapy has been established in this study could increase the percentage of NK -T cells which had the ability to kill tumor cells.Simultaneously ,it is reduced the amount of Treg cells significantly.