CAJ | 학술논문

为监测真空包装鲟鱼在冷藏过程中的优势腐败菌动态变化，通过感官评价和传统选择性培养的方法，对真空包装冷藏鲟鱼中的优势腐败菌进行了分析；同时选择性分离筛选出代表性菌株Q-1和C-1，并基于16S rRNA基因进行了鉴定。结果表明：气单胞菌和肠科菌是真空包装鲟鱼在冷藏过程中的优势腐败菌；菌株Q-1可能为维氏气单胞菌（Aeromonas veronii）新亚种，菌株C-1为路德维希肠杆菌（Enterobacter ludwigii）；同时与菌株C-1相比，菌株Q-1的生长延滞期较长，但生长较快，修正的Gompertz模型能够可靠地分别预测菌株C-1和菌株Q-1在真空包装鲟鱼在冷藏过程中生长动态。
위감측진공포장심어재랭장과정중적우세부패균동태변화，통과감관평개화전통선택성배양적방법，대진공포장랭장심어중적우세부패균진행료분석；동시선택성분리사선출대표성균주Q-1화C-1，병기우16S rRNA기인진행료감정。결과표명：기단포균화장과균시진공포장심어재랭장과정중적우세부패균；균주Q-1가능위유씨기단포균（Aeromonas veronii）신아충，균주C-1위로덕유희장간균（Enterobacter ludwigii）；동시여균주C-1상비，균주Q-1적생장연체기교장，단생장교쾌，수정적Gompertz모형능구가고지분별예측균주C-1화균주Q-1재진공포장심어재랭장과정중생장동태。
The objective of this study was to isolate and identify dominant spoilage bacteria in cold-stored sturgeon under vacuum condition, as well as make predictions of the bacteria growth. Sensory evaluation and selective culture were used to monitor the kinetic growth of dominant spoilage bacteria. Based on 16S rRNA gene sequencing, Q-1 and C-1 were identified and isolated selectively as dominant spoilage bacteria. The results showed that Aeromonas sp. and Enterobacter sp. were dominant spoilage bacteria in cold-stored sturgeon under vacuum condition. Q-1 was likely to be a new subspecies of Aeromonas veronii, and C-1 was Enterobacter ludwigii. Compared with C-1, the lag phase of Q-1 was longer and the growth rate was higher. A modified Gompertz equation could make a credible prediction of the dynamic growth of C-1 and Q-1 in the cold-stored sturgeon under vacuum condition.