CAJ | 학술논문

目的：探讨骨髓间充质干细胞(bone marrow stem cells,BMSCs)通过不同移植途径对治疗兔肝硬化模型疗效的差别。方法用四氯化碳液体橄榄油混合液皮下注射和乙醇喂养的方法建立肝硬化兔模型,将肝硬化模型随机分为肝硬化模型组(n=10)、肝动脉移植组(n=10)、耳静脉移植组(n=10)。取健康新西兰大耳兔骨髓,利用全骨髓贴壁的方法原代培养骨髓 BMSCs,然后通过3种途径分别进行移植并检测血清白蛋白、转氨酶；通过 H E染色观察肝硬化程度以及利用Western-Blotting 法检测血清白蛋白在肝脏中的表达。结果分别通过静脉组与动脉组移植 BMSCs 到兔肝硬化模型中,4周后发现移植组模型兔的肝功能均得到明显改善,血清白蛋白较肝硬化未移植组显著升高(P<0.05),转氨酶较肝硬化未移植组显著降低(P<0.05)。2种途径移植BMSCs对兔肝硬化的治疗效果有统计学差异(P<0.05)。结论 BMSCs 移植通过耳静脉和肝动脉治疗肝硬化兔4周后,肝功能都有明显的改善。但经肝动脉移植治疗效果明显优于经静脉移植治疗效果。
목적：탐토골수간충질간세포(bone marrow stem cells,BMSCs)통과불동이식도경대치료토간경화모형료효적차별。방법용사록화탄액체감람유혼합액피하주사화을순위양적방법건립간경화토모형,장간경화모형수궤분위간경화모형조(n=10)、간동맥이식조(n=10)、이정맥이식조(n=10)。취건강신서란대이토골수,이용전골수첩벽적방법원대배양골수 BMSCs,연후통과3충도경분별진행이식병검측혈청백단백、전안매；통과 H E염색관찰간경화정도이급이용Western-Blotting 법검측혈청백단백재간장중적표체。결과분별통과정맥조여동맥조이식 BMSCs 도토간경화모형중,4주후발현이식조모형토적간공능균득도명현개선,혈청백단백교간경화미이식조현저승고(P<0.05),전안매교간경화미이식조현저강저(P<0.05)。2충도경이식BMSCs대토간경화적치료효과유통계학차이(P<0.05)。결론 BMSCs 이식통과이정맥화간동맥치료간경화토4주후,간공능도유명현적개선。단경간동맥이식치료효과명현우우경정맥이식치료효과。
Objective To investigate the effect of bone marrow mesenchymal stem cells (bone marrow stem cells,BMSCs) through different approaches in the treatment of transplanted cirrhotic model in rabbit effect difference.Methods Establishment of rabbit model of carbon tetrachloride cirrhosis with liquid olive oil mixture of subcutaneous inj ection and ethanol fed,the liver cirrho-sis models were randomly divided into cirrhosis model group (n=10),hepatic artery transplantation group (n=10),ear vein trans-plantation group (n=10).Healthy New Zealand rabbits bone marrow,using the method of whole bone marrow adherent cultured bone marrow BMSCs,and then were transplanted and serum albumin,alanine aminotransferase in 3 ways;by HE staining,the de-gree of hepatic cirrhosis and detection of serum albumin by Western blot expression in liver.Results Through the vein group and ar-tery transplantation group BMSCs to cirrhotic model in rabbit,4 weeks after the discovery of liver transplantation group rabbits were significantly improved,serum albumin is liver cirrhosis without transplantation group increased significantly(P<0.05),transami-nase is liver cirrhosis without transplantation group decreased significantly (P<0.05).The therapeutic effect of 2 ways of BMSCs transplantation on rabbit liver cirrhosis had a significant difference (P<0.05).Conclusion Transplantation of BMSCs through the ear vein and the hepatic artery of rabbit after 4 weeks of treatment of liver cirrhosis,liver function can be improved.But the hepatic artery transplantation therapy is obviously better than that of intravenous transplantation of treatment effect.